Here we evaluated the use of internal reference compounds for the rapid assessment of reactions performed in solid-phase. An internal reference compound (commercially available) was bound to the resin, together with the substrate, and cleaved with the products after completion of the reaction. The peak area of the reference compound in the HPLC-UV chromatograms can be correlated directly with t...

Liquid chromatography combined with mass spectrometry (LC-MS) analysis has been successfully employed in proteome research. Data generated from LC-MS equipments usually comes with a considerable amount of noise. Thus, there is a need for the use of filters to enhance the quality of mass spectrometry chromatograms. An algorithm developed recently (CODA[2]) achieves good results in removing noise...

Summary: Chromaligner is a tool for chromatogram alignment to align retention time for chromatographic methods coupled to spectrophotometers such as high performance liquid chromatography and capillary electrophoresis for metabolomics works. Chromaligner resolves peak shifts by a constrained chromatogram alignment. For a collection of chromatograms and a set of defined peaks, Chromaligner align...

We report on an unexpectedly high rate of unreadable chromatograms from plasmid sequencing using Beckman Coulter's protocols, chemistry, and CEQ8000 instrument. Failed or poor quality plasmid sequence chromatograms were accompanied by a sharp drop, fluctuation, or steady decline in the current and a corresponding delay in signal counts beyond the time of capillary injection. We observe a correl...

GRADIENT ION CHROMATOGRAPHY WAS USED FOR THE SEPARATION OF EIGHT SUGARS: arabitol, cellobiose, fructose, fucose, lactulose, melibiose, N-acetyl-D-glucosamine, and raffinose. The separation method was optimized using a combination of simplex or genetic algorithm with the isocratic-to-gradient retention modeling. Both the simplex and genetic algorithms provided well separated chromatograms in a s...

I review the methodology of "high-performance" liquid chromatography as applied to therapeutic drug monitoring. Aside from direct injection of sample, sample cleanup involves miscible and immiscible organic solvent extractions, solid-phase extraction, and size separations. Column considerations are bonded phases, column dimensions, particle size, guard columns, stability, pH range, and reproduc...

Dissolved organic matter (DOM) in the transition zone from freshwater to marine systems was analyzed with a new approach for parameterizing size distribution of compounds. We used size-exclusion chromatography molecular analysis and quantified colored DOM (CDOM) on samples two coastal environments Baltic Sea (Roskilde Fjord, Denmark Gulf Gdansk, Poland). applied Gaussian decomposition method id...

A simplified method for the preparation of the Dittmer-Lester spray for the detection of phospholipids on thin-layer chromatograms is described.