Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro...

Whole-tissue quantification at single-cell resolution has become an inevitable approach for further quantitative understanding of morphogenesis in organ development. The feasibility of the approach has been dramatically increased by recent technological improvements in optical tissue clearing and microscopy. However, the series of procedures required for this approach to lead to successful whol...

Tissue optical clearing technique provides a prospective solution for the application of advanced optical methods in life sciences. This paper gives a review of recent developments in tissue optical clearing techniques. The physical, molecular and physiological mechanisms of tissue optical clearing are overviewed and discussed. Various methods for enhancing penetration of optical-clearing agent...

In recent years, advances in laser microscopy and endogenous fluorescent tagging techniques have led to the development of many tissue-clearing strategies, which render tissues optically transparent, allowing large blocks of unsectioned tissue to be visualized in three dimensions (3D). CLARITY (Clear Lipidexchanged Acrylamide-hybridized Rigid Imaging/ Immunostaining/In situ hybridization-compat...

A f r a m e w o r k f o r m o d e l i n g a n d r e c o g n i t i o n o f t e m p o r a l a c t i v i t i e s is proposed . T h e m o d e l i n g o f s e t s o f e x e m p l a r a c t i v i t i e s is ach ieved by p a r a m e t e r i z i n g t h e i r r e p r e s e n t a t i o n in t h e f o r m o f p r i n c i p a l c o m p o n e n t s . R e c o g n i t i o n o f s p a t i o t e m p o r a l v ...

Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolut...

This study tests four hypotheses related to forest clearing over time in Ecuador's northern Amazon: (1) a larger increase in population over time on a farm (finca) leads to more deforestation; (2) rates of forest clearing surrounding four primary reference communities differ (spatial heterogeneity); (3) fincas farther from towns/communities experience lower rates of forest clearing over time; a...