نتایج جستجو برای: cloning efficiency
تعداد نتایج: 450287 فیلتر نتایج به سال:
The transformation-associated recombination (TAR) cloning technique allows selective and accurate isolation of chromosomal regions and genes from complex genomes. The technique is based on in vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or hooks, to the gene of interest. The recombination occurs during transformation of yeast spheroplasts that ...
BACKGROUND Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction sites and/or ligation. Here, we describe a simple and fast method for performing gene reconstitution by modified RF cloning. RESULTS Double-stranded inserts and acceptors were ...
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced. High abortion and fetal mortality rates are commonly observed. These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. Various strateg...
We describe a simple electroelution method for purifying large, gel-fractionated DNA molecules that alleviates the need for melting of the agarose and subsequent enzymatic agarose digestion. The method yields DNA that is visibly more intact than that purified from a standard agarose-digestion protocol and is more amenable to large-fragment cloning with PAC and BAC vectors. These findings are no...
Methods are presented for the improved yield and analysis of blunt-ended cloning of PCR-generated DNA fragments. We show that Pfu DNA polymerase polishing of Taq DNA polymerase-generated fragments increases the yield and efficiency of cloning. Using a triple primer set consisting of two outside, asymmetrically distanced primers and one fragment-specific primer, both the presence and orientation...
BACKGROUND Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells is currently the most widely used strategy to generate tailored pig models for biomedical research. Although this approach facilitates a similar spectrum of genetic modifications as in rodent models, the outcome in terms of live cloned piglets is quite variable. In this study, we aimed at a comprehensive an...
In the new millennium, traditional electrical power systems have undergone a significant change driven by set of requirements arising from evolving and changing technology. Thus, fundamental changes occurred in way energy is produced, transmitted, distributed. This situation has revealed need to expand existing networks or establish networks. The available literature that particular attention l...
U937 cells, an established monoblast or early monocyte cell line, were assessed as a model in vitro for the regulation of cell growth at the single cell level. Colony formation by 500 U937 cells, preinduced to a state of responsiveness to lactoferrin (LF) by incubation with human y Interferon was suppressed by LF. LF-suppressed colony formation was restored by partially purified growth activity...
Recently Galvão and Hardy have shown that quantum cloning can improve the performance of some quantum computation tasks. However such performance enhancement is possible only if quantum correlations survive the cloning process. We investigate preservation of the quantum correlations in the process of non–local cloning of entangled pairs of two–level systems. We consider different kinds of quant...
In this paper, the authors investigate the prospect of using multi-resolution histograms (MRH) in conjunction with digital image forensics, particularly in the detection of two kinds of copy-move manipulations, i.e., cloning and splicing. To the best of the authors’ knowledge, this is the first work that uses the same feature in both cloning and splicing forensics. The experimental results show...
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