The strain of Penicillium janthinellum NCIM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24h followed by UV-irradiation for 3min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-d-glucose (SM3). These mutants were...

A bacterium, isolated from rabbit’s waste and identified as Cellulomonas sp., had cellulase and thermostable α-amylase activity when grown on wheat bran. Maximum activity of thermostable α-amylase was obtained by adding 3% soluble starch. However, soybean oil (1 ml l) could increase the production of α-amylase and cellulase in wheat bran. The α-amylase was characterized by making a demonstratio...

The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55C, respectively....

Objective: Peel of Amorphophallus is considered as waste. In this study we checked for the presence of cellulase enzyme in this waste and investigated its biochemical properties. Methods: Presence of cellulase activity was confirmed by Congo red plate diffusion assay The optimum temperature, pH, substrate concentration for both CMCase and FPase activity was also determined by DNS method. Effect...

Aspergillus niger KIJH was grown in solid and submerged fermentation using leaves roots (with without bark) of plants typically from Brazilian semiarid as substrate to produce a multienzymatic extract, which characterised for its potential biotechnological applications. Solid-state (SSF) applied select the most promising biomass induction substrates production hydrolytic enzymes by fungus. The ...

Production of reducing sugar by hydrolysis of corncob material with Streptomyces sp. cellulase and ethanol fermentation of cellulosic hydrolysate was investigated. Cultures of Streptomyces sp. T3-1 improved reducing sugar yields with the production of CMCase, Avicelase and β-glucosidase activity of 3.8, 3.9 and 3.8 IU/ml, respectively. CMCase, Avicelase, and βglucosidase produced by the Strepto...

A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on S...

Cellulases are enzymes used in the hydrolysis of lignocellulosic residues for second-generation (2G) bioethanol production, low availability market, and high-cost impacts 40% total cost 2G production. Therefore, it is necessary to look sustainable alternatives its mainly produced by Aspergillus niger, their activity can be affected nitrogen concentration, use surfactants, carbon source, among o...