The enoyl reductase from Mycobacterium tuberculosis (MtInhA) was shown to be a major target for isoniazid, the most prescribed first-line anti-tuberculosis agent. MtInhA (EC 1.3.1.9) protein catalyzes hydride transfer 4S hydrogen of β-NADH carbon-3 long-chain 2-trans-enoyl thioester substrates (enoyl-ACP or enoyl-CoA) yield NAD+ and acyl-ACP acyl-CoA products. latter are long carbon chains mero...

La aorta es la arteria más grande y de mayor importancia del cuerpo humano, encargada llevar sangre rica en oxigeno desde el corazón a todas las demás zonas organismo. Cuando ocurre un estrechamiento esta arteria, se produce lo que llaman coartación aortica, ésto hace tenga bombear con fuerza para poder impulsar través aorta. Es tipo afección congénito detecta edad adulta presenta conducto arte...

coronary heart disease is the leading cause of death and disability in adults. the association between acute coronary syndrom (acs) and elevated serum high sensitivity c-reactive protein (hscrp) suggests that chronic inflammation of the coronary arterial wall may play an important role. a number of drugs used in the treatment of cardiovascular disease reduce serum crp. it* is therefore possible...

Chain elongation and cyclization of precursors of dihydroxyphenylacetyl-CoA (DPA-CoA) catalyzed by the bacterial type III polyketide synthase DpgA were studied. Two labile intermediates, di- and tri-ketidyl-CoA (DK- and TK-CoA), were proposed and chemically synthesized. In the presence of DpgABD, each of these with [(13)C(3)]malonyl-CoA (MA-CoA) was able to form partially (13)C-enriched DPA-CoA...

Cytoplasmic acetoacetyl-CoA thiolase (acetyl-CoA C-acetyltransferase, EC 2.3.1.9) was partially purified from rat liver. The enzyme was irreversibly inactivated by 4-bromocrotonyl-CoA, but-3-ynoyl-CoA, pent-3-ynoyl-CoA and dec-3-ynoyl-CoA. In the case of the alk-3-ynoyl-CoA esters the potency as alkylating agents of acetoacetyl-CoA thiolase decreased with increased chain length of the alk-3-yno...

The mitochondrial metabolism of 5-enoyl-CoAs, which are formed during the beta-oxidation of unsaturated fatty acids with double bonds extending from odd-numbered carbon atoms, was studied with mitochondrial extracts and purified enzymes of beta-oxidation. Metabolites were identified spectrophotometrically and by high performance liquid chromatography. 5-cis-Octenoyl-CoA, a putative metabolite o...

In a previous study, the essential role of 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase acyl-CoA dehydrogenase (Acd) in Advenella mimigardefordensis strain DPN7(T) (AcdDPN7) during degradation of 3,3'-dithiodipropionic acid (DTDP) was elucidated. DTDP is a sulfur-containing precursor substrate for biosynthesis of polythioesters (PTEs). AcdDPN7 showed high amino acid sequence similarity t...

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that member of the Firmicutes. It often positive for catalase and nitrate reduction. Pathogenic strains promote infections by producing virulence factors such as potent protein toxins, expression cell-surface binds inactivates antibodies. Sepsis-related to methicillin-resistant S. (MRSA) has significant morbidity high mortality ra...