Neutrophil granulocytes rely on a functional actin network for directed migration. Microtubule disassembly does not impair receptor-linked chemotaxis, instead it induces development of polarity and chemokinesis in neutrophils concomitant with polarized distribution of alpha-actinin and F-actin. Cells stimulated with colchicine, which disassembles microtubules, migrate with a speed comparable to...

Colchicine was used as chemical mutagen for In vitro induction of polyploids. Nodal segments of In vitro grown marigold plantlets were cultured on MS medium supplemented with four different concentrations of colchcine (0.0%, 0.001%, 0.01% and 0.05%) for 12h, separately. After treatment, nodal segment were shifted to colchicine free MS medium for normal growth. Highest mortality rate (72.74%) an...

Previously we reported negative terata and c-mitosis synergism of FireMaster (polybrominated biphenyls) with colchicine in subacutely treated rats. Now we report absence of chromosome aberrations from FireMaster and absence of c-mitosis synergism of FireMaster and colchicine in male mice. For the study of chromosome aberrations groups of three mice received 0, 50, or 500 mg/kg FireMaster or 4.5...

Localization of alkaline phosphatase (ALP) and cathepsin D (CAPD) in primary cultures of fetal rat hepatocytes was examined using double immunofluorescent staining in order to investigate the relationship between lysosome movement and the fate of ALP during cell restoration after microtubule disruption by colchicine. At 3 hr and 24 hr after colchicine treatment, numerous coarse dots containing ...

Colchicine is beneficial in the treatment of cirrhotic patients, it prevents changes in plasma membrane bound enzymes induced by CCl4 intoxication. In this study, lipid composition and microviscosity were measured in liver plasma membranes isolated from rats given CCl4. Microviscosity values increased in rats given CCl4 for six weeks but fell considerably in those given CCl4 for 10 weeks. Both ...

We read with great interest the paper by Solomon et al on the effects of colchicine on risk of cardiovascular events and mortality among patients with gout. The authors should be commended on a well-planned and informative study, which, despite the non-randomised retrospective design, adds valuable evidence on a potential new role of this old drug in cardiovascular disease—a prospect which has ...

BACKGROUND Patients with severe developmental disabilities often have concurrent decreased neuromuscular tone of the gastrointestinal tract, which can lead to a weak gag reflex, esophageal reflux, aspiration, and severe intractable constipation. High doses of multiple laxatives are frequently needed to maintain bowel motility in these patients. Colchicine, a natural alkaloid that is primarily u...

It is conventional to divide the management of gout into the treatment of the acute attacks, the interim treatment, and treatment of the tophaceouy stage.' It is probably fair to say that, at least until 1950, virtually everything we did in the management of patients with gout was empiric. The use of colchicine developed on a purely empiric basis and has a respectable and fascinating history; t...

Microtubules have been shown to alter contraction in cardiac myocytes through changes in cellular stiffness. However, an effect on excitation-contraction coupling has not been examined. Here we analyze the effects of microtubule disruption by 1 micromol/L colchicine on calcium currents (I(Ca)) and [Ca(2+)](i) transients in rat ventricular myocytes. I(Ca) was studied using the whole-cell patch-c...