نتایج جستجو برای: cold adapted

تعداد نتایج: 181909  

2005
THOMAS W. MOON P. W. HOCHACHKA

1. The kinetics of the thermally induced enzyme variants of the supernatant NADP-isocitrate dehydrogenase from rainbow-trout liver are investigated. 2. Fish acclimatized to 20C (cold-adapted enzyme) and 170C (warm-adapted enzyme) show different relative distributions of the three NADP-isocitrate dehydrogenase isoenzymes; this has been demonstrated with electrophoresis and electrofocusing techni...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1993
I Dey C Buda T Wiik J E Halver T Farkas

The compositions and physical states of the liver phospholipids of marine and freshwater fish adapted to relatively constant but radically different temperatures were investigated. Fish adapted to low temperature (5-10 degrees C) accumulated more unsaturated fatty acids than those in a warm (25-27 degrees C) environment. There were no measurable differences in the gross fatty acid compositions ...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2003
M Lucassen A Schmidt L G Eckerle H-O Pörtner

Adjustments in mitochondrial properties and capacities are crucial in acclimatization to seasonal cold and in evolutionary cold adaptation of marine ectotherms. Although long-term compensatory increments in aerobic capacity of fish tissues have frequently been described in response to cold, much less is known about transitional phases and gene expression patterns involved. We investigated the t...

2015
Zi-Chao Yu Bai-Lu Tang Dian-Li Zhao Xiuhua Pang Qi-Long Qin Bai-Cheng Zhou Xi-Ying Zhang Xiu-Lan Chen Yu-Zhong Zhang Israel Silman

Although the Escherichia coli expression system is the most commonly used expression system, some proteins are still difficult to be expressed by this system, such as proteins with high thermolability and enzymes that cannot mature by autoprocessing. Therefore, it is necessary to develop alternative expression systems. In this study, a cold-adapted Pseudoalteromonas expression system was develo...

2013
Marcus Fornbacke Mats Clarsund

Proteases have been used in medicine for several decades and are an established and well tolerated class of therapeutic agent. These proteases were sourced from mammals or bacteria that exist or have adapted to moderate temperatures (mesophilic organisms); however, proteases derived from organisms from cold environments-cold-adapted or psychrophilic proteases-generally have high specific activi...

Journal: :Infection and immunity 1984
Y Z Ghendon F I Polezhaev K V Lisovskaya T E Medvedeva G I Alexandrova A I Klimov

A previously described cold-adapted attenuated virus, A/Leningrad/134/17/57 (H2N2), was further modified by 30 additional passages in chicken embryos at 25 degrees C. This virus had a distinct temperature-sensitive (ts) phenotype, grew well in chicken embryos at 25 degrees C, and failed to recombine with reference ts mutants of fowl plague virus containing ts lesions in five genes coding for no...

2013
Renaud Berlemont Olivier Jacquin Maud Delsaute Marcello La Salla Jacques Georis Fabienne Verté Moreno Galleni Pablo Power

An Antarctic soil metagenomic library was screened for lipolytic enzymes and allowed for the isolation of a new cytosolic esterase from the a/b hydrolase family 6, named MHlip. This enzyme is related to hypothetical genes coding esterases, aryl-esterases and peroxydases, among others. MHlip was produced, purified and its activity was determined. The substrate profile of MHlip reveals a high spe...

2016
Zaida Ortega Abraham Mencía Valentín Pérez‐Mellado

Alpine lizards living in restricted areas might be particularly sensitive to climate change. We studied thermal biology of Iberolacerta cyreni in high mountains of central Spain. Our results suggest that I. cyreni is a cold-adapted thermal specialist and an effective thermoregulator. Among ectotherms, thermal specialists are more threatened by global warming than generalists. Alpine lizards hav...

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