نتایج جستجو برای: commercial elisa

تعداد نتایج: 182018  

اعتمادی, افشار, بوترابی, سید مهدی, حبیبی, غلام رضا, صراف نژاد, عبدالفتاح, فلاحی, شیرزاد, محمدی, محسن, میرجلیلی, علی,

Background: Anti-dsDNA antibodies frequently found in the sera Systemic Lupus Erythematosus patients, particularly in active disease stage. Nowadays exploit different eukaryotic and prokaryotic dsDNA as antigen source and different reagents as binder. The aim of this study to compared two dsDNA different sources and tow different kinds of reagents for binder in ELISA test. Methods: In this stu...

H. Dadras H. Ghadakchi S.A. Pourbakhsh, S.M.H. Hosseini

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Gh. Moghaddam H. Jabbari Nooghabi P. Khazraiinia R. Jafari Jozani,

The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...

2015
Elke Albrecht Frode Norheim Bernd Thiede Torgeir Holen Tomoo Ohashi Lisa Schering Sindre Lee Julia Brenmoehl Selina Thomas Christian A. Drevon Harold P. Erickson Steffen Maak

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We hav...

2014
Eleonora Ponterio Ilaria Di Bartolo Ginevra Orrù Manuel Liciardi Fabio Ostanello Franco Maria Ruggeri

BACKGROUND The hepatitis E virus (HEV) has been detected in both humans and animals, particularly pigs, worldwide. Several evidences, including human infection following consumption of raw contaminated meat, suggest a zoonotic transmission of HEV. In Italy, large circulation of genotype 3 HEV has been reported in swine, and recent studies have confirmed the involvement of this genotype in autoc...

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