INTRODUCTION In this protocol, sample and competitor RNAs (previously synthesized as described in Preparation of Competitor RNA for Competitive RT-PCR) are reverse transcribed (separately) in a pilot experiment. A constant amount of sample RT product is then combined with a 2-logserial dilution of competitor RT product for PCR. This procedure provides an approximate copy number for the sample, ...

introduction butyrivibrio fibrisolvens strains are presently recognized as the major butyrate-producing bacteria found in the rumen and digestive track of many animals and also in the human gut. in this study we reported the development of two dna based techniques, quantitative competitive (qc) pcr and absolute based real-time pcr, for enumerating butyrivibrio fibrisolvens strains. despite the ...

Aim—To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). Methods—The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T gondii. An artificial competitor containing the four primer binding sites was made first by creating...

The convergence of wireless networks, affordable sensors and embedded computing enables a new physical Internet, which will revolutionize our everyday life, from dinning to traveling. In North America, the new terms are coined such as ThingsThat-Think, Sensor Web, or Smart Dust. In Europe, many people call it Ambient Intelligence (AmI), which includes two essential components: perception and co...