The multidrug resistance P-glycoprotein (P-gp) was recently proposed to redistribute cholesterol in the plasma membrane, suggesting that P-gp could modulate cholesterol efflux to cholesterol acceptors. To address this hypothesis and to reevaluate the role of P-gp in cholesterol homeostasis, we first analyzed the role of P-gp expression on cholesterol efflux in P-gp stably transfected drug-selec...

Present study investigated the possible role of P-glycoprotein in intracellular exposure of active substance (robinin) natural medicine Flaroninum™ and effect robinin on cellular accumulation mitoxantrone either BCRP-overexpressing BCRP-negative cells. Robinin - kaempferol-3-O-b-D-robinoside-7-O-α-L-rhamnopyranoside, has been obtained from over ground parts Astragalus falcatus Lam., growin...

By screening a chemical library for the compounds protecting cells from adriamycin (Adr), a series of small molecules was isolated that interfered with the accumulation of Adr in mouse fibroblasts by enhancing efflux of the drug. Isolated compounds also stimulated efflux of Rhodamine 123 (Rho-123), another substrate of multidrug transporters. Stimulation of drug efflux was detectable in the cel...

introduction: gutta-percha (gp), is a neutral and non-toxic material. the aim of this animal study was to compare the biocompatibility of nanosilver coated gp (ns-gp) with conventional gp in subcutaneous tissues in a rat model. methods and materials: conventional gp and ns-gp were subcutaneously implanted in the backs of 20 male wistar rats ( n =10). a control animal was assigned for each trial...

Multidrug resistance (MDR) is one of the major obstacles to efficient chemotherapy against cancers, resulting from overexpression drug efflux transporters such as P-glycoprotein (P-gP). In present study, we aimed evaluate MDR reversal activity and synergistic therapeutic potential cationic anticancer peptide L-K6 with doxorubicin (DOX) on P-gP-overexpressing DOX-resistant MCF-7/ADR human breast...

This paper examines determinants of creditor recoveries from defaulted debt instruments. First, we argue that to properly measure a debt instrument’s relative position in a firm’s debt structure, debt pari passu to the instrument must be accounted for. We thus propose a new measure of seniority and find that it is the most important determinant of recovery rates. Second, firm-level variables, e...

Flavonoids are constituents of fruits, vegetables, and plant-derived beverages, as well as components in herbal-containing dietary supplements. The objective of this investigation was to characterize the effect of flavonoids on P-glycoprotein (P-gp)-mediated cellular efflux and to determine the molecular mechanism(s) of the flavonoid-drug interaction. Studies were conducted in the sensitive and...

We have investigated the involvement of P-glycoprotein (P-gp)/caveolin-1 interaction in the regulation of brain endothelial cells (EC) migration and tubulogenesis. P-gp overexpression in MDCK-MDR cells was correlated with enhanced cell migration whereas treatment with P-gp inhibitors CsA or PSC833 reduced it. Transfection of RBE4 rat brain endothelial cells with mutated versions of MDR1, in the...