In this paper, we address a new technique for automatic construction of steel structure in high-rise buildings termed RCA system (Robotics & Crane based Automated Construction System). RCA system can be divided into four core systems: 1) Monitoring and control system, 2) Material assembly system, 3) Beam assembly system, 4) Construction Factory (CF) system. Through our research, we expect that ...

Obtaining full-length cDNA is important for many molecular biology methods like cDNA library construction or RACE-PCR (rapid amplification of cDNA ends). We have found that the inclusion of betaine alone and in combination with trehalose in reverse transcription results in longer cDNA synthesis products. As shown on the 14-kb long clarithrin mRNA with real-time PCR, a combination of 2 M betaine...

This paper provides a new advanced probabilistic approach for reliable estimation of the fatigue characteristic S-N curves of welded joints both for constant amplitude (CA) and variable amplitude (VA) loading conditions. The presented approach, which is referred to as the ML-MCS approach, combines Maximum Likelihood method (ML) and Monte-Carlo Simulations (MCS) method to estimate true p-quantil...

Judicious and regulated use of wood as a building material is better than that of many other conventional materials in terms of environmental issues of this century. The study of the behavior of wood requires a better understanding of the characteristics in different possible cases of loading including loads applied instantly, loads applied for a short time and loads applied for a long time. Th...

The advent of massive parallel sequencing (MPS) has revolutionized the field of human molecular genetics, including the diagnostic study of mitochondrial (mt) DNA dysfunction. The analysis of the complete mitochondrial genome using MPS platforms is now common and will soon outrun conventional sequencing. However, the development of a robust and reliable protocol is rather challenging. A previou...

Our current understanding of Antarctic soils is derived from direct culture on selective media, biodiversity studies based on clone library construction and analysis, quantitative PCR amplification of specific gene sequences and the application of generic microarrays for microbial community analysis. Here, we investigated the biodiversity and functional potential of a soil community at Mars Oas...

Polyphasic studies that used phospholipid fatty acid analysis (PLFA) in conjunction with community level physiological profiling (CLPP) or PCR-based molecular methods were analyzed in order to evaluate the power of each strategy to detect treatment effects on soil microbial community structure (MCS). We found no studies where CLPP or PCR-based methods differentiated treatments that were not als...

A central challenge in sequencing single-cell genomes is the accurate determination of point mutations, phasing of these mutations, and identifying copy number variations with few assumptions. Ideally, this is accomplished under as low sequencing coverage as possible. Here we report our attempt to meet these goals with a novel library construction and library amplification methodology. In our a...

In contrast to most other building materials the strength of glass is not a material parameter, but rather a combination of the material treatment and the load position effects. Structural glass elements are produced with heat treatment when compressive residual stresses are created on the surface. The loading position of glass specimen is also significant due to variations in material quality....