نتایج جستجو برای: cortical culture
تعداد نتایج: 381231 فیلتر نتایج به سال:
abstract seizure disorders are the most common neurological illnesses in infants and children. presented is an 8 year old boy with nocturnal vomiting episodes, found to have eeg characteristics of early onset benign occipital epilepsy, better known as panayiotopoulos syndrome.
Recent studies in rodents have suggested that some cortical GABAergic interneurons arise within the neuroepithelium of the subcortical telencephalon then migrate dorsally into the cerebral cortex. These studies have relied heavily on short-term organotypic culture methods and on the analysis of mutant mice that die during the neonatal period. The purpose of this study is to ascertain directly w...
Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cumulus cells, granulosa cells, Ovarian Mesenchymal Cells (OMC) and Oviductal Epithelial Cells (OEC) was studied. Large preantral follicles were isolated by microdissecting the trypsin (1%) digested ovarian cortical slices. Cumulus cells were isolated by repeated pipetting of oocytes, granulosa cells were iso...
the effect of high voltage, high frequency pulsed electromagnetic field on slain ovine cortical bone
background: this study is the first research investigating the possible effect of pulsed power signals on the characteristics of cortical bone by comparing the mechanical properties of this type of bone pre and post expose to pulsed power. methods: a positive buck-boost converter was applied to generate adjustable high voltage, high frequency pulses (up to 500 v and 10 khz). the functional beha...
BACKGROUND Little is known about the factors that control the initiation of growth of primordial follicles. Primordial follicles in pieces of fetal bovine ovarian cortex spontaneously activate in vitro and develop to the primary stage, but few follicles develop further. For decades, embryologists have grafted tissue to the chorioallantoic membrane (CAM) of chick embryos to study the development...
In the neurological mutant mouse reeler, the histological organization of the neocortex develops abnormally and essentially results in an inversion of the relative positions of the cortical layers. The reeler mutation, therefore, provides an insight into the molecular mechanisms underlying the formation of the cortical layers. We have generated a monoclonal antibody (CR-50) that probes a distin...
Caloric restriction is an anti-aging intervention known to extend lifespan in several experimental models, at least in part, by stimulating autophagy. Caloric restriction increases neuropeptide Y (NPY) in the hypothalamus and plasma ghrelin, a peripheral gut hormone that acts in hypothalamus to modulate energy homeostasis. NPY and ghrelin have been shown to be neuroprotective in different brain...
Neurons in "mature" (4- to 6-week-old) dissociated cell cultures of 15-day gestational age rat fetal cortex were injected with Lucifer Yellow in order to compare their detailed morphological features with those of cortical neurons in situ, and in order to determine which features of cellular morphology were dependent on local environmental conditions. Neurons were characterized by their cell fo...
We assayed glutamate transport activity in cultures of rat cortical neurons containing < 0.2% astrocytes. Using [3H]L-glutamate as the tracer, sodium-dependent high affinity glutamate transport was demonstrated [K(m) = 17.2 +/- 2.4 microM; Vmax = 3.3 +/- 0.32 nmol/mg of protein/min (n = 5)]. Dihydrokainate (1 mM) inhibited uptake of radioactivity by 88 +/- 3% and had a Ki value of 65 +/- 7 micr...
The neurotoxicity of 3 non-NMDA glutamate receptor agonists--kainate, alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA), and quisqualate--was investigated quantitatively in dissociated murine cortical cultures. Five minute exposure to 500 microM kainate, but not AMPA, produced widespread acute neuronal swelling. Kainate-induced swelling was resistant to 2-amino-5-phosphonovalerate (A...
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