A new method is developed for the determination of acrylamide in starch-based foods. The method included the extraction of acrylamide with water, defatting with hexane, derivatization with potassium bromate (KBrO(3)) and potassium bromide (KBr), liquid-liquid extraction with ethyl acetate-hexane (4:1), and concentration. The final analyte (2-bromopropenamide, 2-BPA) is analyzed by high-performa...

The goal of this work was to compare Pressurised Liquid Extraction (PLE), which is usually used to extract drugs from soil, and a modified-QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. Furthermore, several clean-up methods were evaluated. Selective Pressurised Liquid Extraction (SPLE) and Dispersive Solid Phase Extraction (dSPE) used after PLE and QuEChERS, respectively, wer...

Ned Tijdschr Klin Chem Labgeneesk 2008, vol. 33, no. 3 Introduction 5-Hydroxyindole-3-acetic acid (5-HIAA) is the most abundant metabolite of serotonin (5-hydroxytryptamine: 5-HT). The neurotransmitter/neurohormone serotonin is synthesized from the essential amino acid tryptophan in the enterochromaffin cells of the gut and in serotonergic neurons in the central nervous system (1). Peripheral s...

Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...

A new transdermal delivery system that controls cellulitis is evaluated using reversed-phase high-performance liquid chromatography coupled with photodiode array detection. An extraction procedure and the validation of the analytical method to assay the active excipients from the Centella asiatica plant (asiaticoside, madacessic acid, and asiatic acid) are described. Excellent results ae obtain...

We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. ...

A rapid method has been developed for extracting neutral lipid from small samples of human adipose tissue, with a petroleum ether (“Skellysolve B”) as the extracting solvent. It is coupled with a simple method of transesterification, to convert the purified triglycerides to fatty acid methyl esters. Virtually no phospholipid is extracted by the petroleum ether. Analysis of the extracted lipid b...

Pigments of the flavanol-anthocyanin (F-A+) type detected earlier in wine are synthesized using a protocol adapted from the synthesis of procyanidin dimers. The F-A+ adduct thus obtained is purified by countercurrent liquid-liquid partition, currently referred to as countercurrent chromatography (CCC). The solvent system consists of tert-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5,...

Ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-QTOF/MS) based on a chemical profiling method was applied to rapidly evaluate the chemical consistency between sun-dried and sulfur-fumigated Radix Paeoniae Alba. By virtue of the high resolution, high speed of UPLC, and the accurate mass measurement of TOFMS coupled with reliable MarkerLynx software, fiv...

Liquid chromatography coupled with mass spectrometry detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, rapid method development. In this context, a liquid chromatography-tandem mass spectrometry method has been deve...