To understand the evolution of Ulva chloroplast genomes at intraspecific and interspecific levels, in this study, three complete compressa Linnaeus were sequenced compared with available cpDNA data. Our comparative analyses unveiled many noticeable findings. First, genome size variations cpDNAs levels mainly caused by differences gain or loss group I/II introns, integration foreign DNA fragment...

Chilling injury in cucumber (Cucumis sativus L.) is conditioned by maternal factors, and the sequencing of its chloroplast genome could lead to the identification of economically important candidate genes. Complete sequencing of cucumber chloroplast (cp)DNA was facilitated by the development of 414 consensus chloroplast sequencing primers (CCSPs) from conserved cpDNA sequences of Arabidopsis (A...

Relationships among the five groups of extant seed plants (cycads, Ginkgo, conifers, Gnetales, and angiosperms) remain uncertain. To explore relationships among groups of extant seed plants further and to attempt to explain the conflict among molecular data sets, we assembled a data set of four plastid (cpDNA) genes (rbcL, atpB, psaA, and psbB), three mitochondrial (mtDNA) genes (mtSSU, coxI, a...

Olive oil traceability remains a challenge nowadays. DNA analysis is the preferred approach to an effective varietal identification, without any environmental influence. Specifically, olive organelle genomics is the most promising approach for setting up a suitable set of markers as they would not interfere with the pollinator variety DNA traces. Unfortunately, plastid DNA (cpDNA) variation of ...

The chloroplast is an essential plant organelle responsible for photosynthesis. Gene duplication, relocation, and loss in the chloroplast genome (cpDNA) are useful for exploring the evolution and phylogeny of plant species. In this study, the complete chloroplast genome of Paris verticillata was sequenced using the 454 sequencing system and Sanger sequencing method to trace the evolutionary pat...

Jack pine (Pinus banksiana) and lodgepole pine (Pinus contorta var. latifolia) are two North American boreal hard pines that hybridize in their zone of contact in western Canada. The main objective of this study was to characterize their patterns of introgression resulting from past and recent gene flow, using cytoplasmic markers having maternal or paternal inheritance. Mitochondrial DNA (mtDNA...

Few studies of cpDNA have provided evolutionary and/or phylogenetic information at the intraspecific level. We analyzed restriction site variation using 19 endonucleases in 37 populations representing both diploid (2n = 14) and autotetraploid (2n = 28) Tolmiea menziesii. Seven restriction site mutations and five length mutations were observed. Although diploid and tetraploid Tolmiea have been i...

The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. The chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. After...

a restriction-site analysis of chloroplast dna (cpdna) was carried out to evaluate the levelof diversity in vicia faba l. germplasm selected from different geographical regions using 11 restrictionendonucleases. we analyzed 214 restriction sites in 18 accessions of vicia faba. all of the accessionshad identical cpdnas, pointing out the ancestral character of all the accessions are of only one g...

This work aims to analyze the phylogeography of Schizolobium parahyba(Fabaceae), which includes two varieties with a disjunct distribution, from Southern Brazil to Central America. Neotropical rain forests, focus on four largest wet forests: Atlantic forest, Amazonian forests, Andean forest and Central America forest. The genetic diversity and differentiation of populations among S. parahyba po...