A natural rsbU mutant of Staphylococcus aureus, unable to activate the alternative transcription factor s via the RsbU pathway and therefore forming unpigmented colonies, produced first-step teicoplanin-resistant mutants upon selection for growth in the presence of teicoplanin, of which the majority were of an intense orange color. By using an asp23 promoter-luciferase fusion as an indicator, t...

A regional hybrid variational–ensemble data assimilation system (HVEDAS), the maximum likelihood ensemble filter (MLEF), is applied to the 2011 version of the NOAA operational Hurricane Weather Research and Forecasting (HWRF) model to evaluate the impact of direct assimilation of cloud-affected Advanced Microwave Sounding Unit-A (AMSU-A) radiances in tropical cyclone (TC) core areas. The forwar...

The Joint Polar Satellite System (JPSS) mission has provided over ten years of high-quality data products for environment forecasting and monitoring through the current Suomi National Polar-orbiting Partnership (S-NPP) NOAA-20 satellites. Particularly, sensor record (SDR) derived environmental (EDR) from Visible Infrared Imaging Radiometer Suite (VIIRS), Cross-track Sounder (CrIS), Advanced Tec...

Staphylococcus aureus is an important human commensal and opportunistic pathogen responsible for a wide range of infections. Long chain unsaturated free fatty acids represent a barrier to colonisation and infection by S. aureus and act as an antimicrobial component of the innate immune system where they are found on epithelial surfaces and in abscesses. Despite many contradictory reports, the p...

Staphyloxanthin is a virulence factor which protects Staphylococcus aureus in stress conditions. We isolated two pigment variants of S. aureus and one strain of Pseudomonas aeruginosa from a single wound infection. S. aureus variants displayed white and yellow colony phenotypes. The sequence of the operons for staphyloxanthin synthesis indicated that coding and promoter regions were identical b...

A fully connected deep neural network (FCDN) clear-sky mask (CSM) algorithm (FCDN_CSM) was developed to assist the FCDN-based Community Radiative Transfer Model (FCDN_CRTM) reproduce Visible Infrared Imaging Radiometer Suite (VIIRS) radiances in five thermal emission M (TEB/M) bands. The model design referenced and enhanced from its earlier version (version 1), trained tested global ocean domai...

Global Navigation Satellite System (GNSS) Radio Occultation (RO) data play an essential role in improving numerical weather prediction (NWP) and monitoring climate change. The NOAA Commercial RO Purchase Program (CDP) purchased provided by Spire Inc. To ensure the quality from is consistent with other missions, we need to quantify their accuracy retrieval uncertainty carefully. In this work, We...

Diabetic foot infection (DFI) is a common complication in diabetes patients, with infections being the leading cause of amputations. Staphylococcus aureus frequently found diabetic infections, which methicillin-resistant (MRSA) has become major clinical and epidemiological challenge. Since MRSA strains are resistant to most β-lactam antibiotics, also partially other treatment difficult costly. ...

This paper presents a before-after crash, injury and traffic study of constructing bicycle tracks and marking bicycle lanes in Copenhagen, Denmark. Corrections factors for changes in traffic volumes and crash / injury trends are included using a general comparison group in this non-experimental observational study. Analysis of long-term crash trends points towards no significant abnormal crash ...