© Translational Andrology and Urology. All rights reserved. Transl Androl Urol, 2015;4(S1) www.amepc.org/tau were divided into four equal aliquots: (A) untreated, (B) diluted in cryoprotectant, (C) diluted in cryoprotectant and cryopreserved for 2 days and (D) diluted in cryoprotectant and cryopreserved for 2 months. The DNA methylation degree of H19 and MEST ICR was determined by Bisulfite Seq...

INTRODUCTION The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of rabies virus using different cryopreser...

Cryopreservation would potentially very much facilitate the inventory control and distribution of laboratory-produced organs and tissues. Although simple freezing methods are effective for many simple tissues, bioartificial organs and complex tissue constructs may be unacceptably altered by ice formation and dissolution. Vitrification, in which the liquids in a living system are converted into ...

Abstract Due to fast relaxation processes of transition metal ions, electron paramagnetic resonance (EPR) spectroscopy metalloproteins needs be performed at cryogenic temperatures. To avoid damaging the biological system upon freezing, a cryoprotectant is generally added sample as glassing agent. Even though cryoprotectants are expected inert substances, evidences in literature show their non-i...

Surimi is stabilized myofibrillar proteins that have been blended with cryoprotectant for a longer frozen storage life. The functional properties of the myofibrillar proteins are protected during frozen storage when a cryoprotectant was added. Some commonly used cryoprotectants are sorbitol, sucrose, polydextrose, lactitol, litesse, maltodextrin, trehalose, sodium lactate and mixtures of the ab...

Hematopoietic stem cell infusion is an important procedure in Hematopoietic Stem Cell Transplantation (HSCT). This study identifies transfusion and other adverse reactions that can occur during infusion and the nursing care related to the procedure. This epidemiologic study used transplantations performed between 2006 and 2008. A total of 166 transplantations were performed: 114 were autologous...

We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were s...