The aim of this work was to evaluate the variability of the as1-casein locus in the endangered Girgentana dairy goat breed in order to define genetic improvement and a conservation program for this breed. The study was performed on 200 dairy goats by means of different PCR protocols. Themost frequent alleles were A (0.590) and F (0.290) followed by B (0.065) and N (0.047).CSN1S1 E allele was id...

Genetic variations in the four casein genes CSN1S1 , CSN2 CSN1S2 and CSN3 have obtained substantial attention since they affect milk protein yield, composition, cheese processing properties, digestibility as well tolerance human nutrition. Furthermore, variants are used for breed characterization, biodiversity, phylogenetic studies. The current study aimed at identification of five domestic goa...

The COP9 signalosome (CSN) is involved in multiple developmental processes. It interacts with SCF ubiquitin ligases and deconjugates Nedd8/Rub1 from cullins (deneddylation). CSN is highly expressed in Arabidopsis floral tissues. To investigate the role of CSN in flower development, we examined the expression pattern of CSN in developing flowers. We report here that two csn1 partially deficient ...

Selective signatures in whole genome can help us understand the mechanisms of selection and target causal variants for breeding program. In present study, we performed Extended Haplotype Homozygosity (EHH) tests to identify significant core regions harboring such signals in Chinese Holstein, and then verified the biological significance of these identified regions based on commonly-used bioinfo...

This study has established a successful protocol to cryopreserve lumpfish Cyclopterus lumpus (Linnaeus, 1758) milt. Three cryosolutions were tested based on Mounib's medium; the original medium including reduced l-glutathione (GSH), the basic sucrose and potassium bicarbonate medium without GSH, or with hen's egg yolk (EY). Dimethyl sulphoxide (DMSO) was used as the cryoprotectant along with al...