introduction: hepatitis c virus (hcv) infection is a major public health problem which influences about 170 million people worldwide. different types of vaccines have been designed using hcv structural proteins to control the viral infection. the core nucleocapsid protein is one of the most conserved proteins and a desirable target for hcv vaccines, especially the protein-based vaccines. in cur...

background prostate cancer is a serious genetic disease known as the first widespread cancer in men, yet the molecular changes required for the cancer progression is not fully understood. availability of high-throughput gene expression data has led to the development of various computational methods for the identification of critical genes involved in the cancer. methods in this paper, we show ...

objective(s): streptococcus pyogenes produces extracellular hyaluronidase enzyme. this enzyme is directly associated with the spread of the organism during infection. the objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. materials and methods: the enzymatic region of hyaluronidase gene was detecte...

introduction: antimicrobial peptides as the body's defense strategy play an important role in resistance against infection of microorganism. these peptides are able to modulate the immune and inflammatory processes through the production of defensive molecules. therefore, the modulatory effects of brevinin-2r, an antimicrobial peptide extracted from the skin of the frog (rana ridibunda), w...

B acteriophage can convert their bacterial host from a nonpathogenic form to a pathogenic form by providing the bacterium with virulence genes, in a process called lysogenic phage conversion. Vibrio cholerae is a bacterium prevalent in marine environments that can infect humans to cause the devastating diarrheal disease cholera, which is endemic in much of Asia and Africa. Although the health a...

pollen from mesquite (prosopis juliflora) is one of the important causes of immediate hypersensitivity reactions in the arid and semi-arid regions of the world. the aim of present study is to produce and purify the recombinant form of allergenic ole e 1-like protein from the pollen of this allergenic tree. immunological and cross-inhibition assays were performed for the evaluation of ige-bindin...

background and objectives: baculovirus can be used as a vector in gene delivery system. viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. in this regard, the gene coding for a subunit of shiga toxin (stxa) from escherichia coli ( e. coli ) strain was cloned in a baculovirus expression system. stxa subunit has the ab...

background: botulinum neurotoxin (bont) complexes consist of neurotoxin and neurotoxin-associated proteins. hemagglutinin-33 (ha-33) is a member of bont type a (bont/a) complex. considering the protective role of ha-33 in preservation of bont/a in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. thus in this study, ha-33 was expr...