The aim of this study is to identify and update the list viral pathogens in tomato seeds, which plays an important role for our country's economy, control, on behalf country, factors like TYLCV PepMV, were thought not be existing past but determined by new sensitive methods recent years. It also aim, if PepMV are found, share findings with necessary institutions needs tested, quarantine lists. ...

One of the important antiviral genetic strategies used in crop breeding is recessive resistance. Two eukaryotic translation initiation factor 4E family genes, eIF4E and eIFiso4E, are the most common recessive resistance genes whose absence inhibits infection by plant viruses in Potyviridae, Carmovirus, and Cucumovirus. Here, we show that another eIF4E family gene, nCBP, acts as a novel recessiv...

This chapter presents a summary of the results of experiments conducted in Alabama and Florida over a five year period to evaluate strains of plant growth-promoting rhizobacteria (PGPR) for induction of resistance against insect-transmitted diseases on field-grown cucumber and tomato. Experiments with cucumber demonstrated that treatment with PGPR significantly reduced the incidence of wilt sym...

Replication of viral RNA genomes requires the specific interaction between the replicase and the RNA template. Members of the Bromovirus and Cucumovirus genera have a tRNA-like structure at the 3' end of their genomic RNAs that interacts with the replicase and is required for minus-strand synthesis. In Brome mosaic virus (BMV), a stem-loop structure named C (SLC) is present within the tRNA-like...

The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is involved in intercellular virus transport. In this study, putative RNA-binding properties of the PNRSV MP were studied. The PNRSV MP was produced in Escherichia coli using an expression vector. Electrophoretic mobility shift assays (EMSAs) using DIG-labelled riboprobes demonstrated that PNRSV MP bound ssRNA cooperatively wit...

BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

Viral attacks on natural populations of herbaceous forage legumes might have adverse effect on seed and herbage production, hence this study was carried out to investigate the type and incidence of viruses occurring on these plants in Nigeria. Protein A-sandwich (PAS) and antigen-coated plate (ACP) enzyme-linked immunosorbent assays (ELISA) were used to detect ten legume viruses from 100 leaf s...

Gaillardia plants exhibiting symptoms characteristic of viral and phytoplasmal diseases were collected at botanical gardens and floriculture farms in Lithuania. Cucumber mosaic cucumovirus was isolated from diseased plants exhibiting symptoms characterized by stunting, flower breaking and malformation of petals. The virus was characterized based on electron microscopy, serology, reactions of in...