BACKGROUND RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA." A co-culti...

BACKGROUND The use of culture-independent nucleic acid techniques, such as ribosomal RNA gene cloning library analysis, has unveiled the tremendous microbial diversity that exists in natural environments. In sharp contrast to this great achievement is the current difficulty in cultivating the majority of bacterial species or phylotypes revealed by molecular approaches. Although recent new techn...

System of Rice Intensification (SRI) paddy was introduced to offset the heavy cost of Traditional paddy cultivation. To decrease the cost of cultivation in Traditional paddy, to increase profits of the farmers in rice cultivation by decreasing the use of fertilizers, pesticides and minimizing water use by scientific water management in the face of labour scarcity, SRI paddy was introduced in Ma...

Rain-shelter cultivation is an effective cultural method to prevent rainfall damage during grape harvest and widely applied in the Chinese rainy regions. In this study we investigated the effect of rain-shelter cultivation on grape diseases and phenolic composition in the skins of Vitis vinifera cv. Cabernet Gernischet grape berries through the comparison with open-field cultivation at two vint...

With the rapid development of Belt and Road Initiative, cultivate interdisciplinary language talents should be further explored. As International Language Service has became a subdiscipline under Discipline Foreign Literature, cultivation training contemporary service began to accelerate. This paper discussed overall industry, challenges, principles, macro strategy micro approaches cultivating ...

The organization of crop cultivation practices is still far from completion, and Web Resources are not used adequately. This paper proposed a method, based on SVM, to organize the knowledge of crop cultivation practices efficiently from Web Resources. The knowledge organization method of crop cultivation was proposed with Good Agricultural Practices (GAP) in the application of the crop cultivat...