American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

Mycoplasma contaminants can be considered important not only for their roles as pathogens but also they may indicate that insufficient care has been taken during vaccine manufacture or quality control. The purpose of this study was to test the poliomyelitis vaccines for Mycoplasma by culture and polymerase chain reaction (PCR) methods. During 2008 to 2009, a total of 47 lots of oral poliomyelit...

american foulbrood (afb) is the most serious brood disease of the honey bee. traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. the aim of this study is the detection of paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and pcr. therefore 54 samp...

early diagnosis of bacteremia and its complications is the most important part of care and management of the febrile patients. however, a majority of patients who appear to be clinically septic have negative blood culture. the use of polymerase chain reaction (pcr) techniques has allowed identifying the pathogenic organisms rapidly and accurately. the objective of this study was to investigate ...

Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...

an amplification polymerase chain reaction (pcr) test for the direct detection of chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. during 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specimens from ...

background: helicobacter pylori ( pylori ) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. environmental factors such as water a reservoir of h.pylori which infect human.  a non-culture bacteria in coccoid forms widespread in aquatic environments.the objective of this study  was elevating the diagnostic value of pcr and culture methods f...

enteritis due to campylobacter is the most common cause of acute bacterial diarrhea worldwide. in most cases, infection occurs as a result of consuming contaminated water or food, especially raw meat of fowls. campylobacters are saccharolytic and fastidious bacteria. these traits limit the number of available biochemical tests by which isolates may be differentiated. these limitations might, in...

conclusions pcr was the most sensitive, accurate and rapid method for diagnosis of tb. it was concluded that, for the diagnosis of pulmonary tuberculosis, pcr directly from sputum may be recommended in bangladesh, especially in suspected tuberculosis patients who remain negative by conventional methods. patients and methods a total of 150 clinically suspected pulmonary tb patients (male/female:...