background and aims: the aim of this study was to determine the virulence and antibiotic resistance properties of streptococcus agalactiae (s. agalactiae) and streptococcus uberis (s. uberis) isolated from the ruminant’s sub-clinical mastitic milk in iran. materials and methods: positive samples in the cmt test were cultured and s. agalactiae and s. uberis have been identified using biochemical...

background: various reasons accounted for the infection of infants kept at neonatal intensive care unit (nicu). expressed breast milk may be regarded as a source of infection in infants. this study investigated the source of bacterial contamination and the impact of educational interventions on the contamination of mothers’ expressed breast milk (ebm) level whose infants are hospitalized at nic...

Fecal culture has been the primary method used to diagnose paratuberculosis in goats. It is laborious, slow, and expensive. Validation of enzyme-linked immunosorbent assays (ELISAs) on milk samples could make paratuberculosis testing more widely available for goat farmers. The aim of this study was to determine the accuracy of serum and milk ELISAs for paratuberculosis, relative to fecal cultur...

Four lactic acid bacteria (LAB) strains were cultured in reconstituted skim milk with different solids contents to compare the thermotolerance of resulting cultures. As concentration was increased from 5 30 wt%, population non-lactose-fermenting by around 1 log at stationary growth phase. High maintained relative stable pH (?pH<0.5) when fermented a lactose-fermenting strain, compared drastic d...

The objective of the present study was to evaluate the antibiotic susceptibility of 25 Listeria monocytogenes isolates isolated from raw cattle milk towards 15 antibiotics presently used in veterinary and human therapy. Antibiotic susceptibility tests were performed by using the Kirby Bauer disk diffusion method. The isolates were tested for their susceptibility to 15 different common antimicro...

It has been shown previously that cultures of mouse mammary epithelial cells retain their characteristic morphology and their ability to produce gamma-casein, a member of the casein gene family, only if they are maintained on floating collagen gels (Emerman, J.T., and D.R. Pitelka, 1977, In Vitro, 13:316-328). In this paper we show: (a) Cells on floating collagen gels secrete not only gamma-cas...

Antibody detection-based tests for paratuberculosis offer speed and economy, 2 diagnostic test attributes important to animal industries with narrow profit margins. Application of such tests to individual milk samples instead of serum samples can further improve testing efficiency and decrease testing cost. Accuracy of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay (ELIS...