Previous work in this laboratory showed that diethylstilbestrol was capable of suppressing induced furunculosis in rabbits. The present study indicates that the synthetic estrogenic hormone diethylstilbestrol which is used for acne, estrogen deficiency, cancer, and other disorders, can reduce the cytolytic action of staphylococcal alpha toxin. The cytotoxic action of purified alpha toxin for ti...

Infectious bovine rhinotracheitis is an important disease of cattle with serious economic implications. The etiology is bovine herpes virus type-1 which leads to clinical to latent infections and abortions. In this study, an aborted bovine fetus was suspected of IBR infection. The virus was grown in cell line (MDBK) and cytopathic effect observed. CPE began at 12 hr post infection of cells and ...

Int J Vaccines Vaccin 2016, 3(2): 00060 Abbreviations: FMDV: Foot and Mouth Disease Virus; BHK: Baby Hamster Kidney; CPE: Cytopathic Effects; ELISA: Enzyme Linked Immunosorbent Assay; FMD: Foot and Mouth Disease; CFT: Complement Fixation Test, VNT: Virus Neutralization Test; PBS: Phosphate Buffered Saline; IF: Infectious Fluids; OD: Optical Density; BA: Bacteriological Media; ELISA: Enzyme Link...

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for the first autochthonous Italian outbreak in 2007. A226V mutation in E1 has been associated with enhanced replication in A. albopictus vector. Possible involvement of this mutation in enhanced infection capability in primate cells and sensitivity to exogenous interferon (IFN)-a was investigated. No significant differe...

RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells wer...

Four plants having known medicinal properties were screened for inhibition of goatpox virus (GTPV) replication in vitro. Of the 4 plants, extract of Acacia arabica (Babul) and Eugenia jambolana (Jamun) leaves had inhibition (%) 99.70 and 99.92 at their maximum non toxic concentrations, 99.93 +/- 0.38 and 1999.73 +/- 0.50 microg/ml, respectively in all cytopathic effect (CPE) inhibition assays. ...

Ten recent isolates of canine distemper virus (CDV) strains were classified according to the growth ability and development of syncytial cytopathic effects (CPE) in Vero cells. Strains P94S, Ac96I, S124C, MD231, MS232, MSA5 and 095Cr were classified as Type 1 and exhibited hardly and did not develop CPE in Vero cells. Strains 007Lm, 009L and 011C were classified as Type 2 as grew well but faile...

In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

vaccination against mumps is included in world health organization (who) program of global immunization. safety and efficacy of vaccines must be confirmed by control units in charge of public health. in iran, the secondary control on potency of vaccine has not been set up yet. we decided to overcome this problem by developing who and nibsc methods in food and drug control lab. ‎nine dilutions o...