Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the basis of their saccharide-binding activity and cell surface localization. Surpr...

F amilies of molecules often have similar functions—think of kinases, transcription factors, chemokines, or Toll-like receptors. The galectins, an evolutionarily ancient family of carbohydrate binding proteins found in organisms from multicellular fungi to humans, are different (1, 2). Galectins are a family of lectins; the family designation is based on highly conserved structural features, pa...

The vitelline layers (VLs) of unfertilized sea urchin eggs were isolated by homogenization in a hypotonic medium containing Triton X-100 and EDTA. The surface topography of the VL is not changed by isolation. The thickness of the isolated VLs (300-400/~) is greater than that reported for VLs on intact eggs (100-200 .~). Sperm adhere to the isolated VLs. When both internal and external VL surfac...

Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the pathogenic fungi Paracoccidioides brasiliensis and P. lutzii using standard procedures. Total EV carbohydrates were ethanol-precipitated from preparations depleted of lipids and pr...

background: premenstrual syndrome (pms) is one of the most common disorders in women in reproductive age. the study aimed to assess the effect of carbohydrate-rich supplements and diets on premenstrual syndrome. methods: the study was conducted within two stages. first stage was descriptive cross sectional investigation to find out frequency and subjects diagnosed as pms according to the apa cr...

Lysin motif (LysM) is a carbohydrate-binding module often found in secreted or transmembrane proteins living organisms from prokaryotes to eukaryotes. Thus far, all characterized LysM-containing plants are plasma membrane-resident receptors co-receptors playing roles plant–microbe interactions. Here, we interrogate the Arabidopsis LysM/F-box-containing protein InLYP1 and reveal its function gly...

this study was carried out to determine the protein and carbohydrate molecular structure of sorghum cultivars using fourier transform infrared spectroscopy (ftir) with multivariate molecular spectroscopy analyses. sorghum cultivars included: 1- kimia, 2- sepideh, 3- m2 and 4- m8. protein and carbohydrate molecular functional groups studied included: peak area and height amide i, amide ii, α-hel...

Background: Oral squamous cell carcinoma is the sixth most common malignancy in the world today.  ING1b/p33 is a newly-discovered tumor suppressor which enhances p53 activity. Transfer of p33 protein from nucleus to cytoplasmic compartment has been previously reported in leukemias. The objective of this study was to determine the correlation between p33ing1b cytoplasmic transfer and lymph node ...

The mouse immunoglobulin G2 monoclonal antibody (MoAb) 465.12S reacts with a cytoplasmic antigen present in human melanoma cells but not detectable in melanocytes. Indirect immunofluorescent staining of a large number of surgically removed normal adult and fetal tissues with the MoAb 465.12S detected the cytoplasmic antigen in epithelial cells from several organs. The intensity of staining was ...