DNA adenine methylase mutants of Salmonella typhimurium contain reduced amounts of FinP, an antisense RNA encoded by the virulence plasmid pSLT. Lowered FinP levels are detected in both Dam- FinO+ and Dam- FinO- backgrounds, suggesting that Dam methylation regulates FinP production rather than FinP half-life. Reduced amounts of F-encoded FinP RNA are likewise found in Dam- mutants of Escherichi...

In this study, we demonstrated that the methyltransferase activity associated with Dam was essential for attenuation of Aeromonas hydrophila virulence. We mutated aspartic acid and tyrosine residues to alanine within the conserved DPPY catalytic motif of Dam and transformed the pBAD/damD/A, pBAD/damY/A, and pBAD/damAhSSU (with the native dam gene) recombinant plasmids into the Escherichia coli ...

In gravitational Higgs mechanism graviton components acquire mass via spontaneous diffeomorphism breaking by scalar vacuum expectation values. We point out that in the massless limit the resulting theory is not EinsteinHilbert gravity (EHG) but constrained gravity (CG). Consequently, massive solutions in the massless limit must be compared to those in CG (as opposed to EHG). We discuss spherica...

The paper studies a method for numerical simulation of free surface flows of viscous incompressible fluids. The approach is based on the level set method for capturing free surface evolution and features compact finite difference approximations of fluid and level set equations on locally refined and dynamically adapted octree cartesian grids. We consider in detail an extension of staggered grid...

Two major branches of engineering mechanics are fluid mechanics and structural mechanics, with many practical problems involving the effect of the first on the second. An example is the design of an aircraft's wings to bend within reasonable limits without breaking under the action of lift forces exerted by the air flowing over them; another is the maintenance of the structural integrity of a d...

A general method for fluid solid interaction problem simulations has been developed in 3D algorithm using incompressible smoothed particle hydrodynamics (SPH) method. The solid is assumed to be rigid so it can be considered as moving boundaries for fluid. Using repulsive force has been proved to be an efficient boundary treatment for incompressible SPH method before with 2D examples. The advant...

The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescens Sr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenin...

The objective of this study was to evaluate the opinions and attitudes of dentists toward the use of rubber dam during various restorative procedures in their dental practices. A structured questionnaire was designed, and distributed to 300 dentists and those that were fully completed were collected for analysis. The collected informations about the opinions and attitudes of dentists toward the...

BACKGROUND Dams are important to ensure food security and promote economic development in sub-Saharan Africa. However, a poor understanding of the negative public health consequences from issues such as malaria could affect their intended advantages. This study aims to compare the malaria situation across elevation and proximity to dams. Such information may contribute to better understand how ...