background: in this study we have prepared the iga solution from normal human plasma using plasma fractionation and ion exchange chromatography. materials and methods: using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction iii was prepared as a suitable source for iga preparation. then it was treated with caprylic acid for separation of impurities. for enrichment o...

glass plate) was covered by Saran wrap, labeled by hot ink, and exposed 10 min to autoradiography film. The region corresponding to the fragment of interest was cut with a scalpel, the Saran wrap was removed, and the sandwich with the piece of the DEAE paper and Whatman 3MM paper was prepared as for unlabeled DNA fragment. The rest of the procedure was identical to that for the unlabeled DNA fr...

Calf lens protein components separated by DEAE chromatography have been related to the crystallins isolated by the new method. Gamma crystallin is the first fraction eluted from the column, folloioed by the beta crystallin components, and then the alpha crystallin subgroup. Since the DEAE separable components could thus be identified as members of the alpha, beta, or gamma crystallin subgroups,...

A total arrest of cell membrane movement occurred after incubation for 2 h of cultured fibroblasts with the polycation DEAE-dextran. The immobilized cells resumed normal membrane undulations after exposure for 5 min to the polyanion dextran-sulphate. The influence of DEAE-dextran on the electrophoretic mobility, which was seen after 10 min of incubation was also erased by a subsequent incubatio...

Various digests of (32)P-labelled DNA were examined by two-dimensional ionophoresis on cellulose acetate and DEAE-cellulose paper. The products from digestion with pancreatic deoxyribonuclease and Neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. Ionophoresis on ...

The clinical significance of initial DEAE chromatography of glucocorticoid binders in lymphoblastic disease was evaluated in an animal model. Domestic cats and dogs with lymphoblastic disease were treated with prednisone, 2 mg/kg/day, for 14 days, and the outcome of therapy was correlated with DEAE chromatograms of glucocorticoid binders, using 3H-triamcinolone as ligand. Six of 30 animals had ...