Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feed...

We present a silk biomaterial platform with highly tunable mechanical and degradation properties for engineering and regeneration of soft tissues such as, skin, adipose, and neural tissue, with elasticity properties in the kilopascal range. Lyophilized silk sponges were prepared under different process conditions and the effect of silk molecular weight, concentration and crystallinity on 3D sca...

The paper presents the synthesis and performance of an immobilized phospholipase A1 with practical application for oil degumming. The polyvinyl alcohol (PVA) had a number of properties indicating this polymer as a good enzyme carrier. The combination with alginate made a macro-porous structure, evidenced by SEM analyses. When the process time in boric acid solution was 30 minutes, the results r...

A biore actor for the mechanical stimulation of silk based scaffolds has been developed. The bi oreactor simulates the natural movement of the anterior cruciate ligament by applying a biaxial movement on the scaffold. The silk scaffolds are prepared in a way that mimicks the mechanical properties (strength, stiffness) of natural anterior cruciate ligaments. The influence of degumming on the mec...

Lecitase Ultra lipase is an acidic lipase which exhibits maximal activity at pH=5.0. It takes part in the activity towards both phospholipid and triglyceride structures. When the temperature is over 40 °C, the phospholipase activity predominates, and the lipase activity is partly suppressed. It could be applied for the degumming of vegetable oil, and the phosphatides in the oil were easily conv...