The phenomenon of detergent insolubility of bovine hippocampal membranes in Triton X-100 was monitored by estimating the presence of phospholipids in the insoluble pellet. This represents a convenient and unambiguous assay and reports the dependence of the extent of phospholipid solubilization on detergent concentration. The advantage of this approach is its ability to accurately determine the ...

Scrapie prion infectivity can be enriched from hamster brain homogenates by using limited proteolysis and detergent extraction. Purified fractions contain both scrapie infectivity and the protein PrP 27-30, which is aggregated in the form of prion rods. During purification, PrP 27-30 is produced from a larger membrane protein, PrPSc, by limited proteolysis with proteinase K. Brain homogenates f...

Detergent-soluble DNA is the fraction (2-4%) of DNA that is released into the supernate upon mild detergent lysis. It is nonmitochondrial in origin. It labels efficiently with deoxy[3H]ribonucleosides and the labeling is prevented by inhibitors of polymerase alpha and ribonucleotide reductase. In previous publications we have characterized detergent-soluble DNA from splenocytes of immunological...

this study was conducted to determine the chemical composition and degradability characteristics of a pasture plant salsola tomentosa by nylon bags method .the sampling was performed from the northern region of plain's gonabad city in three growth stages (vegetative, flowering and seeding). the samples dried in air, were milled and their chemical composition were determined. to determine the de...

When cell membranes are treated with Triton X-100 or other detergents at 4 degrees C, a nonsolubilized fraction can often be recovered, the "detergent-resistant membranes", that is not found when detergent treatment takes place at 37 degrees C. Detergent-resistant membranes may be related in some cases to membrane "rafts". However, several basic aspects of the formation of detergent-resistant m...

intercropping is considered as a method for increasing yield per unit and stability. in order to evaluate the effects of phosphorous biofertilizer and chemical phosphorous on fodder quality of corn (zea mays l.) and grass pea (lathyrus sativa l.) intercropping, two field experiments were conducted as factorial based on randomized complete block design with four replications at two places simult...

The aim of this research is to compare indigestible internal markers acid insoluble ash (AIA) and acid detergent lignin (ADL) and total collection of faeces (TCF) for apparent digestibility estimation in Caspian horse. Analyses were carried out on wheat straw, alfalfa, barley and maize, for dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (ND...

The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.

In a field trial of three disinfectant-detergent preparations for the surgical scrub Hibiscrub (a 4% chlorhexidine detergent solution) maintained a significantly greater residual antibacterial action on the skin of the gloved hands of an operating team than was obtained with Disadine (povidone-iodine detergent preparation) (P<0.001). In comparison with Phisohex (3% hexachlorophane in detergent ...