نتایج جستجو برای: detergent compatibility

تعداد نتایج: 37625  

Journal: :Journal of structural biology 2003
H J Snijder P A Timmins K H Kalk B W Dijkstra

The structure of the detergent in crystals of outer membrane phospholipase A (OMPLA) has been determined using neutron diffraction contrast variation. Large crystals were soaked in stabilising solutions, each containing a different H(2)O/D(2)O contrast. From the neutron diffraction at five contrasts, the 12 A resolution structure of the detergent micelle around the protein molecule was determin...

Journal: :Protein expression and purification 2009
Chaowei Shi Fang Han Ying Xiong Changlin Tian

In membrane protein biochemical and structural studies, detergents are used to mimic membrane environment and maintain functional, stable conformation of membrane proteins in the absence of lipid bilayers. However, detergent concentration, esp. molar ratio of membrane protein to detergent is usually unknown. Here, a gas chromatography-mass spectrometry selected ion monitoring (GC-MS-SIM) method...

2015
Sarah L. Rouse Mark S. P. Sansom

Structural studies of membrane proteins have highlighted the likely influence of membrane mimetic environments (i.e., lipid bilayers versus detergent micelles) on the conformation and dynamics of small α-helical membrane proteins. We have used molecular dynamics simulations to compare the conformational dynamics of BM2 (a small α-helical protein from the membrane of influenza B) in a model phos...

Journal: :Biophysical Journal 1982

Journal: :Journal of the American Oil Chemists' Society 1957

Journal: :Chemistry and Physics of Lipids 2013

Journal: :Journal of Japan Oil Chemists' Society 1971

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