Histamine intolerance is characterized by a large variety of diffuse symptoms, whichcannot bewell attributed, andwhich cannot be graspedbyclassical clinical diagnostics. The authors present a new, three-phase concept for the diagnosis and therapeutical support of this clinical picture. The steps include a standardized anamnestic questionnaire, clinical and chemical evidence of the diamine oxida...

OBJECTIVE Anorexia nervosa (AN) patients often experience gastrointestinal complications caused by their malnutrition. We hypothesized that intestinal integrity is disturbed in AN. Therefore, the objective of the present study was to investigate serum diamine oxidase (DAO) activity, which is considered to be a clinical indicator of the integrity of the intestinal mucosa, in AN patients. METHO...

1. The oxidation of p-dimethylaminomethylbenzylamine by purified placental diamine oxidase was followed by measuring the change in E250 caused by the production of p-dimethylaminomethylbenzaldehyde. 2. The inhibition of this reaction by substrate analogues such as isothiouronium, guanidinium, dimethylsulphonium and trimethylammonium compounds was extensively studied. 3. The type and degree of i...

• A handeable device containing diamine oxidase and soybean peroxidase was prepared. Enzymes were supported on SiO 2 monoliths prepared using bio-waste derived substances. The can detect biogenic amines in solution with low detection limit. pH dependence stability of enzymatic catalytic activities ascertained. shows a limit comparable to other traditional methods. Biogenic are group important o...

This laboratory has previously shown that, when animals are injected with microgram amounts of high specific activity C4-histamine, at least three radioactive metabolic products can be demonstrated in the urine. Paper chromatograms of the urine in butanol80, ethanol 10, and ammonia 30 showed three radioactive peaks. The following information regarding their identity has been published. Peak 1 i...