In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplas...

Localisation of the diaphorase activity of nicotinamide adenine dinucleotide phosphate (NADPH-d), acting as a marker of nitric oxide synthesis (NOS), was studied in the thymus of the rat, mouse and rabbit. The NADPH-d-active cells observed in the rat thymus were irregular in shape with numerous projections and were located on the boundary between the cortex and the medulla. The NADPH-d--active ...

The effects of cerebral metabolism-improving drugs on NADPH diaphorase activity in the mouse brain were studied, and we found that diaphorase activity in the post-mitochondrial fraction of brain homogenate was enhanced by idebenone in a concentration-dependent manner. Histochemical studies also indicated that diaphorase staining was intensified by idebenone at the same concentration. These resu...

Nicotine is a very widely used drug of abuse, which exerts a number of neurovegetative behavioural effects by interacting with the neuronal nicotinic acetylcholine receptor. Using histochemical analysis (NADPH-diaphorase and Fluoro-Jade B dye), the influence of intraperitoneal administration of nicotine on neurons of the hippocampus in 35-day-old male rats of the Wistar strain was studied. At t...

The pattern of cytochrome oxidase, acetylcholinesterase, and NADPH-diaphorase activity was studied in the supratemporal plane, the posterior part of the superior temporal gyrus, and the insula of normal human brains. Five dark cytochrome oxidase regions were found: (i) on Heschl's gyrus (area TC of von Economo and Koskinas); (ii) on the planum polare (area TC/TG); (iii) posterior to Heschl's gy...

The principal center of the accessory olfactory system is the accessory olfactory bulb (AOB). In primates, simians are divided into two groups, New and Old World monkeys, and the AOB is present in only New World monkeys. The common marmoset (Callithrix jacchus) is a species of New World monkey. Although the morphology of the common marmoset AOB has been demonstrated, the distribution patterns o...

Nitric oxide (NO) has been implicated in neurotoxicity and cerebral blood flow changes in chronic neurodegeneration, but its activity in the mammalian prion diseases has not been studied in detail. Nicotine adenine dinucleotide phosphate (NADPH)-diaphorase (NADPH-d) histochemistry is a simple and robust histochemical procedure that allows localization of the tissue distribution of NO synthases....

The distribution of nitric oxide synthase was investigated in human cerebral blood vessels and brain tissues. NADPH-diaphorase histochemistry, which is a marker for nitric oxide synthase in neurons and endothelial cells, revealed periadventitial nerve fibers in the arteries of the circle of Willis and their cortical branches, as well as the common carotid and subclavian arteries. The fibers wer...

Neurons in the superficial layers of the superior colliculus are key elements in the visual system of rodents since they receive extensive afferent projections from retinal ganglion cells. The NADPH-diaphorase histochemical technique was used to detect differences in neuronal nitric oxide synthase (nNOS) in the superficial layers of the superior colliculus (sSC) of young adult (3 months) and ag...