Protein instability is a major obstacle in the production and delivery of monoclonal antibody-based therapies for cancer. This study presents real-time isothermal differential scanning fluorimetry as an emerging method to evaluate the stability of human immunoglobulin G protein with high sensitivity. The stability of polyclonal human immunoglobulin G against urea-induced denaturation was assess...

The equipment for an ultrasensitive, fast, thin-film differential scanning calorimetry @~TDSC! or nanocalorimetry# technique is described. The calorimetric cell ~;0.30 cm! operates by applying a short ~;10 ms! dc current pulse ~;10 mA! to a thin ~;50 nm! patterned metal strip, which is supported by a thin ~;50 nm! SiNx membrane. The calorimeter operates at high heating rates ~15–200 K/ms! and i...