Shoot organogenesis and plant establishment has been achieved for Balanites aegyptiaca (L.) Del. from excised root explants. Young root explants obtained from 4 weeks old aseptic seedlings were used for the induction of direct shoot regeneration by incorporating various cytokinins either singly or in combination with auxins in Murashige and Skoog (MS) basal medium. Root explants cultured on MS ...

The Arabidopsis ENHANCER OF SHOOT REGENERATION 1 (ESR1) gene is thought to regulate the initiation of in vitro shoot regeneration. ESR1 encodes a transcriptional activator belonging to the ETHYLENE-RESPONSIVE FACTOR family. In this study, we investigated the functions of the C-terminal domain of the ESR1 protein. Effectorreporter assays using the GAL4 DNA-binding domain fused with portions of t...

The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid...

Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellul...

OBJECTIVE To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis (S. dulcis) L. METHODS Explants were inoculated on MS basal medium supplemented with kinetin and 6-benzylaminopurine for shoot bud induction. To enhance the shoot induction, various auxins like 3-indoleacetic acid or 3-indolebutyric acid or α-naphthylacetic acid were tested along with ...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...

Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl₂), and silver thiosulphate (STS). The addition of AVG, CoCl₂, and STS signif...

Polyamines (PAs) are low molecular weight metabolites involved in various physiological and developmental processes in eukaryotic and prokaryotic cells. The cellular PA level is regulated in part by the action of amine oxidases (AOs) including copper diamine oxidases (DAOs) and flavoprotein polyamine oxidases (PAOs). In this study, the isolation and characterization of flavin amine oxidases (FA...

At an early stage of shoot regeneration from calli of Arabidopsis, pre-meristematic cell mounds develop in association with localized strong expression of CUP-SHAPED COTYLEDON (CUC) genes. Previous characterization of root initiation-defective 3 (rid3), an Arabidopsis mutant originally isolated as being temperature-sensitive for adventitious root formation, with respect to shoot regeneration im...

The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...