PURPOSE The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E tes...

background and objective: nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. medicinal plants are also of great importance as antibacterial agents. therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of net...

In the present study, we have encountered about 100% sensitivity to Ciprofloxacin by disc diffusion method but Nalidixic acid resistance was about 89%. Interestingly, 80 out of 93 isolates of Salmonella typhi (86.02%) and 37 out of 38 isolates of Salmonella paratyphi A (97.36%) were Nalidixic acid resistant; MIC for Ciprofloxacin being more than 1 μg/ml. This implies that Nalidixic acid resista...

OBJECTIVES To evaluate the usefulness of cefoxitin when used as a surrogate marker for the detection of methicillin resistance. PATIENTS AND METHODS Eight hundred and seventy-one strains of Staphylococcus aureus, collected from eight tertiary referral centres serving diverse socio-economic populations, were included in the study using NCCLS disc diffusion and the agar dilution methods. RESU...

Previously, we showed that microbial susceptibility to antimicrobials in concentrations non-toxic for human cells in culture could be tested using the wet disc topical antimicrobial assay. In this report, wet disc assay and agar well diffusion assay results were compared testing the susceptibility of Ps. aeruginosa isolates from burn patients to concentrations of Polymyxin B non-toxic for cultu...

PURPOSE To quantify the delayed contrast agent enhancement in the nucleus pulposus of the intervertebral disc by means by T1 relaxation time measurements, and to correlate the enhancement with visual grading of disc degeneration. Diffusion of nutrients through the endplate is a key factor in tissue viability in the intervertebral disc. It can be simulated in vivo using magnetic resonance imagin...

Objectives: Grewia paniculata (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of Grewia paniculata. Materials and Methods: ...

introduction: this study is aimed to compare phenotypic test methods and determine antibiotic susceptibility pattern of ampc beta-lactamase producing uropathogenic escherichia coli and klebsiella pneumoniae in clinical isolates. method: e. coli and k. pneumoniae were identified by standard microbiological procedures. screening of ampc beta-lactamase production was done by using cefoxitin disc (...

BACKGROUND Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS Thre...