To characterize the desmosome components that mediate intercellular adhesion and cytoskeletal-plasma membrane attachment, we prepared whole desmosomes and isolated desmosomal intercellular regions (desmosomal "cores") from the living cell layers of bovine muzzle epidermis. The tissue was disrupted in a nonionic detergent at low pH, sonicated, and the insoluble residue fractionated by differenti...

Abstract Objectives: Extracellular vesicles (EVs) are heterogeneous membrane in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes apoptotic bodies. The purpose this study was to compare the distribution EVs obtained under different conditions differential centrifugation, including ultracentrifugation,...

Abstract Background Exosomes, internal proteins, lipids, and nucleic acids coated by phospholipid bilayer membranes, are one type of small extracellular vesicles, which can mediate cell-cell communication. In recent years, exosomes have gained considerable scientific interest due to their widely applied prospect in the diagnosis therapeutics human animal diseases. this study, we describe for fi...

in this paper, we study the numerical solution of hybrid fuzzy differential equations by using differential transformation method (dtm). this is powerful method which consider the approximate solution of a nonlinear equation as an infinite series usually converging to the accurate solution. several numerical examples are given and by comparing the numerical results obtained from dtm  and  predi...

The EL family of description logics (DLs) has been successfully applied for representing the knowledge of several domains, specially from the bio-medical fields. One of its principal characteristics is that its reasoning tasks have polynomial complexity, which makes them suitable for large-scale knowledge bases. In their classical form, description logics cannot handle imprecise concepts in a s...

In this work, different sizing agent aqueous dispersions based on polyetherimide (PEI) were elaborated in order to improve the interface between carbon fibers and a thermoplastic matrix (PEEK). The dispersions were obtained by the emulsion/solvent evaporation technique. To optimize the stability and the film formation on the fibers, two surfactants were tested at different concentrations, with ...

Background: Platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. Platelet concentrates can be prepared by the apheresis method or from the whole blood using the Buffy-coat or Platelet-rich plasma methods. The purpose of this study was to compare four variations of platelet-rich plasma method based on double...

PURPOSE To evaluate the relationship between electrophysiological, psychophysical, and structural measurements in normal and glaucomatous eyes and to test the hypothesis that there is a continuous structure-function relationship between ganglion cell numbers and visual field sensitivity. METHODS Thirty-four normal subjects and 40 patients with glaucoma were examined with the pattern electrore...

The efficiencies of the various methods used for phage concentration have been compared. The two-phase concentration method (with polyethylene glycol and dextran sulfate) gave maximal recoveries of infectivity for coliphages of the T-even and T-odd series and for ribonucleic acid phages and single-stranded deoxyribonucleic acid phages. Precipitation of phages by acid gave high yields when appli...