BACKGROUND Non-steroidal anti-inflammatory drugs (NSAIDs) act by inhibition of cyclooxygenase-2 (COX-2), which is overexpressed in cancer. The role of COX-2 and apoptosis were evaluated in 9,10-dimethylbenz(a)anthracene (DMBA)-induced lung cancer in rat and chemoprevention with indomethacin, a traditional NSAID and etoricoxib, a selective COX-2 inhibitor. METHODS The animals were divided into...

The formation of DNA adducts from [3H]-7-hydroxymethyl-12-methylbenz(a)anthracene (7-OHM-12-MBA) and [3H]-7,12-dimethylbenz(a)anthracene (DMBA) in the epidermis of Sencar mice was analyzed. Comparison of Sephadex LH-20 chromatographic profiles of DNA samples isolated from mice treated with DMBA or 7-OHM-12-MBA suggested that the DMBA-treated animals contained DNA adduct(s) derived from the furt...

We examined the effect of S-allylcysteine (SAC), a water-soluble garlic constituent, on cytokeratin expression, a sensitive and specific marker for differentiation status during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis in male Syrian hamsters. Hamsters were divided into four groups of six animals each. Animals in group 1 were painted with a 0.5% so...

7,12-Dimethylbenz(a)anthracene (DMBA) is a potent carcinogen that induces immunosuppression of both humoral and cell-mediated immunity in mice and other species. Previous studies have shown that CYP1B1 is required for bone marrow toxicity produced by DMBA in mice. Therefore, the purpose of these studies was to determine whether CYP1B1 was required for spleen cell immunotoxicity. Female C57BL/6N...

We reported earlier that replacement of casein by soy protein in the diets rats exposed to carcinogen, dimethylbenz[a]anthracene (DMBA), not only delayed initiation breast tumor but also had a protective effect against development aggressive tumor. The aim this study was elucidate molecular mechanism which offers these beneficial effects. Tumor developed gavage administration single dose 80 mg/...

The modulation in biochemical status of skin and hepatic tissue at the time point of commencement of promotion stage of skin carcinogenesis in mice and its intervention with aqueous Azadirachta indica leaf extract (AAILE) were investigated. 7,12-Dimethylbenz(a)anthracene (DMBA, 500 nmol/100 ul of acetone) was applied topically for 2 weeks (twice weekly), followed by phorbol-12-myristate-13-acet...

We measured the relative binding of the labeled carcinogen 9,10-dimethyl-l,2-benzanthracene-3H (DMBA-3H) to mouse satellite and main-band epidermal DNA 24 hr after the topical application of the carcinogen to mouse skin. Labeled DMBA-bound DNA was fractionated into satellite and main-band components by composite gel electrophoresis and by isopycnic CsCl centrifugation. With both fractionation m...

The present study was designed to elucidate differences in the mechanism of the induction of mouse skin tumors either by the initiation-promotion regimen or by the complete carcinogenesis process. The protocols used to elicit skin tumors were: (a) by the initiation-promotion regimen, a single application of 0.2 ,umol of 7,12-dimethylbenz[a]anthracene (DMBA) followed by twice-weekly applications...

Aim of the present study was to evaluate the anti-tumor effect of orally administered rosmarinic acid in 7,12-dimethylbenz(a)anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice. Phase I and II detoxication agents, lipid peroxidation byproducts, antioxidants and apoptotic biomarkers were used to assess chemopreventive efficacy of rosmarinic acid in DMBA induced skin carcinogenesis...

Glutathione S-transferases (GSTs) are the products of a multigene family. A well-established function of GSTs is to metabolize carcinogens by catalysing the conjugation of electrophilic substrates to glutathione. Whether placental GST (GST-P) is expressed during the promotion of two-stage hamster buccal-pouch mucosa (HBPM) carcinogenesis was investigated here, using 7,12-dimethylbenz[a]anthrace...