نتایج جستجو برای: dna barcode
تعداد نتایج: 509583 فیلتر نتایج به سال:
More than 2,500 species of copepods (Class Maxillopoda; Subclass Copepoda) occur in the marine planktonic environment. The exceptional morphological conservation of the group, with numerous sibling species groups, makes the identification of species challenging, even for expert taxonomists. Molecular approaches to species identification have allowed rapid detection, discrimination, and identifi...
DNA barcoding in Ceratopogonidae has been restricted to interpreting the medically and veterinary important members of Culicoides Latreille. Here the technique is utilised, together with morphological study, to interpret all members of the family in a select area. Limited sampling from the county of Finnmark in northernmost Norway indicated the presence of 54 species, including 14 likely new to...
Herbal dietary supplements made from saw palmetto (Serenoa repens; Arecaceae) fruit are commonly consumed to ameliorate benign prostate hyperplasia. A novel DNA mini-barcode assay to accurately identify [specificity = 1.00 (95% confidence interval = 0.74-1.00); sensitivity = 1.00 (95% confidence interval = 0.66-1.00); n = 31] saw palmetto dietary supplements was designed from a DNA barcode refe...
The scale of diversity of life on this planet is a significant challenge for any scientific programme hoping to produce a complete catalogue, whatever means is used. For DNA barcoding studies, this difficulty is compounded by the realization that any chosen barcode sequence is not the gene 'for' speciation and that taxa have evolutionary histories. How are we to disentangle the confounding effe...
SUMMARY *Currently, no official DNA barcode region is defined for the Fungi. The COX1 gene DNA barcode is difficult to apply. The internal transcribed spacer (ITS) region has been suggested as a primary barcode candidate, but for arbuscular mycorrhizal fungi (AMF; Glomeromycota) the region is exceptionably variable and does not resolve closely related species. *DNA barcoding analyses were perfo...
Premise of the study Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada. Methods Our study examined 20,816 specimens representing 5076 of 5190 vascular plant ...
Several hematologic malignancies are associated with specific chromosomal translocations. Because of the dispersed distribution, chromosomal breakpoints may be difficult to detect using molecular techniques. We present a new application of a recently developed method, DNA fiber fluorescence in situ hybridization (fiber FISH), which allows direct visualization and mapping of chromosomal breakpoi...
Fungi are ubiquitous and their spores found in the air outside within built environment. We investigated fungal species present a teaching laboratory at Shepherd University. Airborne fungi were captured passively three sites on two different culture media. Viable isolated to pure then identified by DNA barcode using internal transcribed spacer region of ribosomal DNA. Twenty-four isolates order...
We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments wer...
First DNA barcode sequences of Psorosa ferrugatella Turati, 1924 from the Iberian Peninsula is published and compared with other European closely related species. Three different habitats where P. inhabits are described.
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