The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37 °C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity i...

BACKGROUND Treatment of differentiated thyroid cancer usually consists of a total thyroidectomy followed by one or several courses of radioiodine ((131)I). (131)I is known to have deleterious effects on radiation sensitive tissues and irradiation to the testes has been shown after its administration. We investigated effects of such treatment on sperm DNA in a patient with differentiated thyroid...

Hypogonadotropic hypogonadism (HH) is an uncommon cause of male infertility and a congenital or secondary disorder characterized by delayed or absent sexual maturation. Congenital abnormalities leading to HH are usually the consequence of deficient GnRH secretion occurring either in isolation (idiopathic hypogonadotropic hypogonadism (IHH)), or in association with anosmia (Kallmann syndrome; KS...

Oxidative stress (OS) plays an essential role in male infertility aetiology by affecting sperm quality, function, and also the integrity of sperm DNA. The assessment of oxidative stress in semen may be an important tool to improve the evaluation of sperm reproductive capacity. The purpose of this study was the evaluation of any possible relation between the unbalance of oxidative stress caused ...

PURPOSE The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. MATERIALS AND METHODS Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation an...

BACKGROUND Varicocele, the abnormal dilatation of the veins in the pampiniform plexus is commonly seen in infertile patients. In this study, we aim to examine sperm DNA damage after the creation of experimental varicocele in rats and to observe the change of this damage after a varicocelectomy. MATERIALS AND METHODS In this experimental study, a total of 30 adult male Wistar albino rats were ...

Objective: To investigate the effect of sperm DNA fragmentation index (DFI) on the clinical outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods: Infertile couples were prospectively recruited in the Reproductive Center in Jiaozuo Maternal and Child Care Service Centre in Henan, China, from July 2015 to December 2016. All the recruited 228 infertile coup...

Traditional routine semen analysis and biochemical assays are insufficient for the determination of fertility status in individual men. In recent years, clinical evidence has shown that damaged human sperm DNA may adversely affect assisted reproductive outcomes and that the spermatozoa of infertile men possess substantially more DNA damage than the spermatozoa of fertile men. In this study, we ...

The appropriate management for male partners of couples with recurrent pregnancy loss (RPL) or recurrent implantation failure during in vitro fertilization (IVF) remains unclear. In particular, men with normal semen parameters are often ignored because the “bulk semen parameters” appear normal [1]. Despite normal semen parameters, male partners in couples with RPL or recurrent implantation fail...

OBJECTIVE Several publications have established a relationship between sperm DNA damage and male factor infertility, based on data from America, Europe, and Asia. This study aimed to compare the extent of sperm DNA damage in sperm samples from Nigerian men with unexplained infertility and in sperm samples from a fertile group composed of sperm donors who had successfully impregnated a female pa...