Histone ADP-ribosylation was studied using two-dimensional gel electrophoresis after cleavage of the nuclear DNA with nucleases. Modified histones carrying different numbers of ADP-ribose groups form a ladder of bands above each variant histone. Cellular lysates containing unfragmented DNA mainly synthesize mono(ADP-ribosylated) histones. Cleavage of the DNA with either DNase I or micrococcal n...

Telomerase, a ribonucleoprotein, is responsible for maintaining the telomere length and therefore promoting genomic integrity, proliferation, and lifespan. In addition, telomerase protects the mitochondria from oxidative stress and confers resistance to apoptosis, suggesting its possible importance for the surviving of non-mitotic, highly active cells such as neurons. We previously demonstrated...

a graph g = (v,e) with p vertices and q edges is said to be a total mean cordial graph if there exists a function f : v (g) → {0, 1, 2} such that f(xy) = [(f(x)+f(y))/2] where x, y ∈ v (g), xy ∈ e(g), and the total number of 0, 1 and 2 are balanced. that is |evf (i) − evf (j)| ≤ 1, i, j ∈ {0, 1, 2} where evf (x) denotes the total number of vertices and edges labeled with x (x = 0, 1, 2). in thi...

The precise position in a DNA sequence is determined in a wide spectrum of assays, by electrophoretic separation and comparison with size marker sequencing ladders analysed in parallel. Such assays include localisation of transcription initiation sites and a variety of DNA-protein interaction studies. For many purposes, a single lane of a Maxam-Gilbert G+A ladder then provides adequate informat...

To clarify whether lipids deposited in human atheromatous lesions induce apoptosis of vascular smooth muscle cells (SMC) and to identify the main component in deposited lipids responsible for inducing apoptosis, we examined the effect of lipids extracted from human atheromatous lesions on apoptosis of cultured SMC and analyzed the content of cholesterol in the lipids. When lipids extracted from...

Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This article offers a brief perspe...

Apoptosis, a major form of cell death, is characterized by chromatin condensation, a reduction in cell volume and endonuclease cleavage of DNA into oligonucleosomal length fragments. The detection of these fragments by gel electrophoresis, as a DNA ladder, is currently used as the major biochemical index of apoptosis. Here we report that key morphological changes of apoptosis can be dissociated...

Piezoresistivity is a fundamental property of materials that has found many device applications. Here we report piezoresistivity in double helical DNA molecules. By studying the dependence of molecular conductance and piezoresistivity of single DNA molecules with different sequences and lengths, and performing molecular orbital calculations, we show that the piezoresistivity of DNA is caused by...

BACKGROUND AND OBJECTIVES DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce a...