PURPOSE To compare the big-bubble method using air and liquid as medium of separation for Descemet membrane endothelial keratoplasty (DMEK) lenticule preparation in an eye bank. METHODS Donor corneas (n=20) were immersed in liquid [tissue culture medium (TCM)]. Air and liquid was injected using a 25-gauge needle in the posterior stroma or as near to the stroma-Descemet membrane (DM) phase as ...

Study background: Descemet stripping automated endothelial keratoplasty (DSAEK) allows selective replacement of the diseased corneal endothelium. However, DSAEK requires a donor cornea and the worldwide shortage of corneas limits its application. In this review, we introduce our recent work on tissue engineering for DSAEK using cultured human corneal endothelial cells (HCEC) and recently publis...

Case reporting the use of one donor cornea for two transplantation procedures: deep anterior lamellar keratoplasty (DALK) in a case of an imminent corneal perforation caused by herpetic stromal necrosis, and Descemet stripping with endothelial keratoplasty (DSEK) in an eye with pseudophakic bullous keratopathy (PBK). Descemet's membrane (DM), denuded by stromal necrosis, served as the starting ...

BACKGROUND To evaluate main features of donor tissue that may influence clinical outcome or complication rate after deep anterior lamellar keratoplasty (DALK). METHODS Donor tissue parameters of 84 consecutive corneal donor grafts used for big-bubble DALK surgery between June 2011 and December 2014 in 84 eyes of 84 patients with disorders of anterior corneal stroma were correlated to clinical...

Abb. 3. Schematischer Aufbau der Membranmatrix der peritrophischen Membran von Calliphora (Aufsicht), wie er sich ohne Berücksichtigung von Adsorption an der Oberfläche des Membranschlauches zur Zeit ergibt. A = vermutlich Mucopolysaccharidschicht (Glucose-14C-Einbau, 210Pb-Austausch), Querbanden ca. 30—40 /x, Längsbanden ca. 5 — 1— fi. B = vermutlich Proteinschicht (Acetat-14C-, Phosphat-32P-A...

PURPOSE To analyze the outcomes of post-cataract surgery descemetopexy for Descemet membrane detachment using intracameral air injection. SETTING Cornea Department, LV Prasad Eye Institute, Hyderabad, India. DESIGN Interventional case series. METHODS The clinical data of patients who had descemetopexy from August 2010 to February 2011 for Descemet membrane detachment after cataract surger...

Endothelial keratoplasty is at present the gold standard for surgical treatment of corneal endothelial pathologies not associated with significant corneal scar. Tremendous progress has been made in recent years in improving the technology of endothelial keratoplasty techniques, such as descemet stripping automated endothelial keratoplasty (DSAEK) and descemet membrane endothelial keratoplasty. ...

Purpose. To report the use of Descemet stripping endothelial keratoplasty (DSEK) in a patient with keratoglobus and chronic hydrops. Case Report. We describe a case of a 28-year-old man with bilateral keratoglobus and chronic hydrops in the right eye secondary to spontaneous Descemet membrane tear. The patient presented with finger counting (CF) vision, itching, foreign body sensation, and seve...