PURPOSE Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC. METHODS Morphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT-PCR and chang...

AT the present time jaundiced eyes are frequently rejected as sources of donor material for corneal grafting or for vitreous implantation. One reason for not using these eyes is their discoloration (Edwards and Locke, 1960). This investigation was undertaken in the hope that adequate reasons for or against the use of jaundiced eyes would be discovered. The bilirubin content of the ocular fluids...

AIMS The aims of this study were to examine the influence of advanced recipient and donor age on the long term outcome of corneal transplantation. METHODS Records of 1036 penetrating corneal grafts in recipients aged > or = 80 years at surgery (defined as the elderly subset) and 8092 donor corneas used for transplantation were obtained from the Australian Corneal Graft Register database, Kapl...

.co m) . donor corneas that were screened in our study. In conjunction with the established quality control for corneal storage in eye banks, there is potential for these PCR assays to aid in rapid screening of clinical specimens for microsporidial contamination and in the diagnosis of ocular infections. Possible causes of primary graft failure could include poor surgical technique, poor donor ...

Detection of human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) in donor eyes was performed. DNAs were extracted from the uvea, and they were amplified using the polymerase chain reaction (PCR). Amplified viral DNAs were detected with liquid hybridisation and chemiluminescent assay in which no radioactive materials were used. This method was shown to have a...

PURPOSE To assess the expression of MMP (matrix metalloproteinase)-2 and -9 and TIMP (tissue inhibitors of metalloproteinases)-1, -2, and -3 in Sorsby fundus dystrophy. DESIGN Cliniciopathological report. METHODS A donor eye with a confirmed S181C mutation in the TIMP-3 gene and an age-matched normal donor eye were studied using in situ hybridization technique with MMP -2 and -9 and TIMP -1...

BACKGROUND Descemet's stripping automated endothelial keratoplasty (DSAEK) has been shown to have superior refractive and visual results compared with penetrating keratoplasty, but higher rates of primary graft failure (PGF). This paper presents donor and surgical risk factors for PGF in DSAEK cases in Asian eyes. DESIGN Retrospective case-control study. PARTICIPANTS All consecutive patient...

PURPOSE To report and discuss cases of lamellar keratoplasty using corneas obtained during previous penetrating keratoplasty in keratoconus eyes. METHODS Corneal buttons were obtained from 7 keratoconus patients and stored in a preserving solution for 7-60 days (average, 32.4 days) before use. The recipient eyes comprised recurrent pterygium 3 eyes, primary pterygium 1 eye, pseudopterygium 1 ...

PURPOSE The aim of this study was to describe the challenges in Descemet membrane endothelial keratoplasty (DMEK) donor preparations and provide new strategies to achieve success. METHODS A series of 263 consecutive DMEK preparation attempts by a novice surgeon during a corneal fellowship are described. In all cases, the Descemet membrane (DM) and the endothelium were peeled off from the dono...