نتایج جستجو برای: dot blot assay
تعداد نتایج: 279986 فیلتر نتایج به سال:
گزنه دو پایه (urtica dioica l.)، دارای خاصیت درمانی در معالجه بیماریهای مزمن انسان می باشد. در این پژوهش اثر ضد ویروسی عصاره های آبی و الکلی گزنه در مهار ویروسهای بیماری زای ایجاد کننده موزائیک گل رُز در محیط کشت مورد بررسی قرار گرفت. تعداد 530 نمونه از گونه های مختلف رُز دارای علائم موزائیک و یا بدون علائم از مناطق محتلف تهران و حومه جمع آوری شد. نمونه ها توسط آزمونهای سرولوژیک الیزای مستقیم و غ...
AIMS To evaluate different hybridisation techniques to detect and type human papillomavirus (HPV) DNAs amplified by consensus primer polymerase chain reaction (PCR) in biopsy and cytological specimens. METHODS A hybrid capture-immunoassay in microtitre wells was performed to detect HPV sequences amplified by PCR and typed by specific oligoprobes. Consensus primers were used to amplify a seque...
Human monocytic ehrlichiosis is an emerging infectious disease caused by Ehrlichia chaffeensis, a gram-negative obligatory intracellular bacterium closely related to E. canis. The immunoreactive recombinant fusion proteins rP28 and rP30 have become available after cloning and expressing of the 28- and 30-kDa major outer membrane protein genes of E. chaffeensis and E. canis, respectively. Wester...
Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...
Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease
A dot-blot, enzyme-linked immunosorbent method and a latex agglutination test were studied for their abilities to detect Vibrio cholerae serotype O1 in aquatic samples by testing artificially contaminated water as well as samples from natural potential sources. Water samples were preenriched with alkaline peptone and then enriched with Monsur peptone water. For the dot-blot test, enriched cultu...
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