Protein phosphorylation is one of the most common post-translational modifications regulating many cellular processes. The phos-tag technology was combined with two-dimensional zymograms, which consisted of non-reducing IEF PAGE or NEPHGE in the first dimension and high resolution clear native electrophoresis (hrCNE) in the second dimension. The combination of these electrophoresis methods was ...

In this study, for the first time we report on a comprehensive overview of different strategies to hyphenate droplet-based sample handling and preparation with electrophoretic separation in formats (i.e. microchip capillary electrophoresis). Droplet-interfaced electrophoresis is an emerging technique which micro/nanometric droplets are used as bridge carrier target analytes between treatment el...

In this paper a new approach to face recognition is presented that achieves double dimension reduction making the system computationally efficient with better recognition results. In pattern recognition techniques, discriminative information of image increases with increase in resolution to a certain extent, consequently face recognition results improve with increase in face image resolution an...

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The performance of two-dimensional electrophoresis in conventional gels in Cartesian coordinates (2-DE) vs. polar coordinates (2-PE) is here evaluated. Although 2-DE is performed in much longer Immobiline gels in the first dimension (17 cm) vs. barely 7-cm in 2-PE, an equivalent resolving power is found. Moreover, due to the possibility of running up to seven Immobiline strips in the radial gel...

The nonlinear phenomena that occur in the electric double layer (EDL) that forms at charged surfaces strongly influence electrokinetic effects, including electro-osmosis and electrophoresis. In particular, saturation effects due to either dielectric decrement or ion crowding effects are of paramount importance. Dielectric decrement significantly influences the ionic concentration in the EDL at ...

Hydrogels are used for wound treatment, as they may contain one or more active components and protect the bed. Papain is of substances that have been with this purpose, alongside urea. In paper, carboxypolymethylene hydrogels containing papain (2% 10% concentrations) urea (5% concentration) were produced. Physical-chemical stability was performed at 0, 7, 15 30 days 2-8ºC, 25ºC 40ºC, well rheol...

stress is a threatening factor that all living organisms encounter throughout life. depending on the type of stress, there are several mechanisms for keeping body homeostasis to minimize stress effects. brain is an organ which shows high sensitivity to stress conditions. although many studies have shown induced-stress effects on rat embryos, little is known about the mechanisms involved in copi...