Double haploid (DH) teknolojisi son yıllarda gelişmiş ıslah programlarında yaygın olarak kullanılan bir araç haline gelmiştir. Mısır ıslahında in vivo double-haploid yöntemiyle saf hat elde etme, ıslahta zaman ve maliyet yönünden büyük avantaj sağlamakta süresini 6 yıl kadar kısaltmaktadır. Ayrıca daha fazla kendilenmiş hattın kolayca edilmesi ıslahtan edilecek başarı oranını artırmaktadır. Dou...

The current and projected climate change that is represented by increasing temperatures humidity levels irregular rainfall patterns promotes the occurrence of preharvest sprouting (PHS) in wheat. PHS results significant economic losses, globally, which necessitates need for high-yielding cultivars with increased tolerance; hence, this study was conducted. evaluated two doubled-haploid (DH) whea...

Aims: High maize yields in modern well-managed agroecosystems depend on the use of elite varieties and hybrids. Unfortunately, because repeated selection at high fertilizer rates, some beneficial traits, such as interaction with arbuscular mycorrhizal fungi or release organic acid anions for phosphate mobilization attracting microorganisms, might be gradually declining genotypes. However, old f...

Thirty doubled haploid (DH) lines of barley derived from F(1) of a cross between the six-rowed cultivar Pomo and two-rowed cultivar Maresi were examined for susceptibility to Fusarium seedling blight (SB) and head blight (FHB), measured by mycotoxin (nivalenol) content of kernels. RAPD (random amplified polymorphic DNA) polymorphism was analysed by using 53 decamer primers. Amplification produc...

Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific induction cereals. Although vivo system based on OsMTL-edited plants has been established rice (Oryza sativa), DH still limited by other factors, such as identification, which one of essential steps required ...

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), has potential to cause major yield losses in durum wheat. Development of cultivars with improved resistance stem rust is a sustainable option for effective control this disease. This study was conducted identify genomic regions associated Ug99-derived Pgt races using doubled haploid (DH) mapping population produced from cross between ...

Recombination is a vital characteristic for quantitative trait loci mapping and breeding to enhance the yield potential of maize. However, recombination characteristics in globally used segregating populations have never been evaluated at similar genetic marker densities. This study aimed to divulge the characteristics of recombination events, recombinant chromosomal segments, and recombination...

The main objective in this research was the genetic analysis of heterosis in rapeseed at the QTL level. A linkage map comprising 235 SSR and 144 AFLP markers covering 2045 cM was constructed in a doubled-haploid population from a cross between the cultivar "Express" and the resynthesized line "R53." In field experiments at four locations in Germany 250 doubled-haploid (DH) lines and their corre...

The recent development of Affymetrix chips designed from assembled EST sequences has spawned considerable interest in identifying single-feature polymorphisms (SFPs) from transcriptome data. SFPs are valuable genetic markers that potentially offer a physical link to the structural genes themselves. However, most current SFP prediction methodologies were developed for sequenced species although ...

Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the ...