Mitochondria isolated from etiolated shoots of com ( Zen mays), wheat (Tddk aestum, baley (HM "*are), soybean ( Glychie max L. Merr.), and mung bean ( Phasebhis awueus) exhibited a prol-pndent 02 uptake subject to respiratory controL ADP/O ratios with proline as substrate were intermediate between ratios obtained with exogenous NADH and malate + pyruvate as substrates. Isotope studies showed pr...

Since the isolation of hydroxylysine as the picrate from the phosphotungstic acid-precipit>able fraction of gelatin acid hydrolysates by Van Slyke, Hiller, Dillon, and MacFadyen (I), other isolation procedures have been reported (2, 3). The most, recent is t.hat of Sheehan and Bolhofer (4) who used chromatographic: techniques in part to obtain analytically pure hydroxylysine from gelatin.’ The ...

1. Bacilysin, a hydrophilic substance formed by certain aerobic spore-forming bacteria that causes lysis in cultures of growing staphylococci, has been produced in aerated cultures of a strain of Bacillus subtilis (A14). A chemically defined medium was used, which contained glucose, Czapek-Dox salts and ferric iron. Production of bacilysin occurred, after a lag, while the culture was still unde...

In this study, the kinetics for reaction of tert-butanol esterification with acetic acid in presence Dowex 50Wx8 catalyst was investigated. The kinetic experiments were conducted 1000 milliliter vessel at temperatures ranged from 50 - 80 oC, loading 25-50 g/L, and molar ratios to 1/3 – 3/1. rate found increase increasing temperature loading. It also conversion increases as ratio alcohol Non-ide...

This method for assay of polyamines (putrescine, spermidine, and spermine) in large numbers of urine samples is based on preliminary analyte isolation by use of a Dowex 50W column, separation by high-voltage paper electrophoresis, reaction with ninhydrin to detect the separated amines, and subsequent direct assay with a dual-wave-length thin-layer chromatography scanner by the zigzag scanning m...

With the aim of preparing the 16 possible ribodinucleotides derived from the four principal ribonucleotides, we made a kinetic study to determine the optimum conditions for a partial alkaline hydrolysis of RNA. Satisfactory results were obtained when the hydrolysis of RNA (1g.) in 0.2m-sodium hydroxide (100ml.) at 37 degrees was ceased approx. 10min. after the start of the reaction. The relativ...

Calf kidney has been used as a tissue source for the isolation of cobalamin analogues, which are defined here as cobalt-containing compounds of distinctive chromatographic behavior that are extractable from tissues by methods conventionally used to extract cobalamin and which, in radioisotope dilution assays, are more active with R-protein as binder than intrinsic factor and are relatively less...