نتایج جستجو برای: edta extraction
تعداد نتایج: 176281 فیلتر نتایج به سال:
Chloroplast ATPase (CF,) was isolated from spinach, pea and maize thylakoids by EDTA extraction followed by anion-exchange chromatography. CF1 was purified and resolved by HPLC into integral CF1, and CF, lacking the 6 & E subunits: CF1(-6) and CFl(-E). Washing Mono-Q-bound CFl with alcohol-containing buffers followed by elution without alcohol produced the p subunit and in separate peaks CF1( 6...
When Escherichia coli Olll:B4 is exposed briefly to ethylenediaminetetraacetate (EDTA) it releases 30 to 50% of its lipopolysaccharide, determined not only by measuring percentage release of colitose, a sugar unique to this polymer, but also by measuring the amount of phenol-extractable lipopolysaccharide remaining with the cell. The fraction released cannot be significantly increased above 50%...
When Escherichia coli Olll:B4 is exposed briefly to ethylenediaminetetraacetate (EDTA) it releases 30 to 50% of its lipopolysaccharide, determined not only by measuring percentage release of colitose, a sugar unique to this polymer, but also by measuring the amount of phenol-extractable lipopolysaccharide remaining with the cell. The fraction released cannot be significantly increased above 50%...
Single and sequential extractions are current useful tools for estimating the availability of metals in soils or sediments. Many chemical extraction procedures have been proposed literature, making comparison difficult. This study compares data consistency potential given by four commonly found literature: two single (using dilute HCl EDTA as reactant) (the modified standardized 3-steps procedu...
This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside of the caps. The reaction is carried out at 65°C during 40 minutes. Calcein allows direct detectio...
The oxygen radical absorbance capacity (ORAC) of bilberry (Vaccinium myrtillus) powder and dried nettle (Urtica dioica) seed powder was studied. In addition, the affect of the following parameters in the ORAC analysis were investigated: 1) extraction solvent (acetone/water, ethanol and acidic methanol), 2) extract dilution, and 3) addition of ethylenediaminetetraacetic acid disodium salt hydrat...
Abstract Ethylenediaminetetraacetic acid disodium (EDTA) can form stable complexes with many metals. To improve the leaching rate of gold and consumption thiosulfate, EDTA ammonia were combined copper ions to a copper-EDTA-ammonia-thiosulfate system. Electrochemical methods used study effect ion, EDTA, polarization voltage. The results showed that increasing concentrations ions, promoted dissol...
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