Mucin staining can be used to evaluate secretory activity of human airways. However, mucin epitopes may be masked by physicochemical properties of the secretions. The aim of this investigation was to examine the effects of the calcium chelator, ethyleneglycol-bis-(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) on the detection of M1/MUC5AC mucin in isolated human bronchial preparations...

Reyes FD, Walters ET. Long-lasting synaptic potentiation induced by depolarization under conditions that eliminate detectable Ca signals. J Neurophysiol 103: 1283–1294, 2010. First published December 30, 2009; doi:10.1152/jn.00704.2009. Activity-dependent alterations of synaptic transmission important for learning and memory are often induced by Ca signals generated by depolarization. While it ...

BACKGROUND Although mouse spermatozoa can be freeze-dried without losing their reproductive capacity, the technique needs further improvements to reduce the incidence of chromosomal damage to spermatozoa. Effects of freeze-drying on human spermatozoa are unknown. METHODS Mouse spermatozoa were suspended in a Tris-buffered EGTA solution briefly (10 min at 37 degrees C) or for 1-7 days at 4 deg...

Activity-dependent alterations of synaptic transmission important for learning and memory are often induced by Ca(2+) signals generated by depolarization. While it is widely assumed that Ca(2+) is the essential transducer of depolarization into cellular plasticity, little effort has been made to test whether Ca(2+)-independent responses to depolarization might also induce memory-like alteration...

Opsonization of clinical isolates of B. fragilis and B. thetaiotaomicron with the six isolated proteins of the alternative complement pathway under physiological conditions resulted in considerable C3 deposition on the bacterial surfaces. The time course of C3 deposition was similar to that observed in EGTA-serum; however, the magnitude of C3 deposition was twofold greater in EGTA-serum. Opsoni...

The protein with the most predominant Ca2+ binding activity in membrane fractions of the human placenta was purified to electrophoretical homogeneity. This protein was identified as the membrane bound form of calmodulin based on the immunological cross reactivity and a number of biochemical properties. The membrane fraction of the human placenta contained 8.3% of the total cellular calmodulin a...

A numerical investigation of the structure and extinction of n-heptane/air partially premixed  ames in a counter ow conŽ guration is reported. The  ame is computed using a detailed reaction mechanism consisting of 275 elementary reactions and 41 species. The mechanism is validated by comparing its predictions with measurements for both the premixed and nonpremixed  ames. The partially premi...