The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR).  By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...

Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV. In this study we report the development of an immunocapture real-time RT-PCR (IC-real-time RT-PCR) assay for direct detection of TMV in soils without RNA isolation. A series of TMV infected leaf sap dilutions of 1:101, 1:...

MJ Martin, Met Office, Exeter, UK. M Balmaseda, ECMWF, Reading, UK L Bertino, NERSC, Bergen, Norway P Brasseur, LEGI, Grenoble, France G Brassington, Bureau of Meteorology, Melbourne, Australia J Cummings, NRL, Monterey, USA Y Fujii, JMA/MRI, Tsukuba, Japan DJ Lea, Met Office, Exeter, UK J-M Lellouche, Mercator Ocean, Toulouse, France K Mogensen, ECMWF, Reading, UK P Oke, CSIRO, Hobart, Austral...

In this paper, we employ the view model given by Bertino to propose a new design approach for a secure multi-level object-oriented database system. The central idea is to provide the user with a multi-level view derived from a single-level secure object-oriented database. Hence the database operations performed on the multi-level views are decomposed into a set of operations on the single-level...

Hakkari ili domates ve biber üretim alanlarında lekeli solgunluk virüsü (Tomato spotted wilt virus, TSWV) yonca mozaik (Alfalfa mosaic AMV)’nün belirlenmesi için yapılan sürveyler 2020 döneminde gerçekleştirilmiştir. Domates sararma, nekroz, şekil bozukluğu simptomu gösteren 184 bitkiden yaprak örneği toplanmıştır. Toplanan örneklerinde TSWV AMV’nin Double antibody sandwich enzyme linked immuno...

The objective of this study was to screen for antigens of the hepatitis C virus (HCV) to establish a new double antibody sandwich-lateral flow immunoassay (DAS-LFIA) method for testing the presence of anti-HCV antibodies in human serum or plasma. A series of different recombinant HCV proteins in Escherichia coli cells were constructed, expressed, purified and the new DAS-LFIA strip was develope...

elisa data is usually handled in the ms excel worksheets through different methods and different formulas. accurate and precise calculations of the data is time consuming and tedious. elisa analyzer was developed with a user friendly interface to streamline titration and analysis of the data obtained from elisa and improve rapid and lucid presentation of the results. the software can also help ...