Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g s...

We established a new highly sensitive method, PCR-ELISA, for the dectection of Babesia bovis in cattle for farms in Thailand. The detection of around 2.4 x 10(-8)% parasitemia (equivalent to 1 infected erythrocyte per 2 ml) was achieved by PCR amplification followed by the ELISA detection of a biotin tagged gene. When comparing the sensitivity of PCR-ELISA with the microscopic method, our PCR-E...

An enzyme-linked immunosorbent assay (ELISA) was developed for detection of bovine serum antibodies to the cytotoxin (leukotoxin) of Pasteurella haemolytica. A partially purified, cytotoxic, and immunogenic protein obtained from supernatants of logarithmic-phase P. haemolytica was used as the ELISA antigen. Preadsorption of sera with various cytotoxic, somatic, and capsular antigen preparations...

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits'...

h.pylori infection stimulates immune responses. these responses at the mucosal level are predominantly of iga types, while circulating antibodies against this microorganism are predominantly igg classes. igm antibodies are rarely found and seem to be non-specific for this bacterium. in this research, water extract antigen, from three strains of h.pylori (isolated from patients with gastritis, d...

Serum antibody detection tests and a urine antigen detection technique were compared in samples from 116 patients epidemiologically characterized as belonging to a legionellosis outbreak. Sera were tested by enzyme-linked immunosorbent assays (ELISAs) for immunoglobulin M (IgM) and IgG plus IgM and by immunofluorescent assays (IFAs) for IgG, IgM, IgA, and polyimmunoglobulin using commercial kit...

Fllahi Sh1, Ghorvi MJ2, Qara-gozlou B3, Sepahvand A1, Mahouti F4 1. Instructor, Department of Parasitology, Faculty of Medicine, Lorestan University of Medical Sciences 2. Associate Professor, Department of Parasitology, Faculty of Para-medicine, Iran University of Medical Sciences 3. Instructor, Department of Immunology, Faculty of Para-medicine, Iran University of Medical Sciences 4. B.Sc of ...

Chlamydial group antigen was extracted from Chlamydia trachomatis strain SA2(f) and used as the antigen for an ELISA. The assay was reproducible since chlamydial antibody titres differed by no more than twofold when sera were tested on up to eight occasions. In tests on sera from 75 patients attending venereal disease or rheumatology clinics, the results of the ELISA and of a microimmunofluores...

Trichomoniasis is a common sexually transmitted disease with an estimated incidence of 4 million to 8 million cases a year in the United States. The most commonly used method of diagnosis is a direct microscopic observation (wet mount) of vaginal secretions and, although both rapid and inexpensive, the sensitivity of this technique is generally 50 to 70%. We developed an indirect enzyme-linked ...