neurotrauma is one of the main medical problems that affect considerable number of population especially young persons in all countries. neurotrauma not only is a serious threat for life but also creates various disabilities and consequently imposes large costs to society. following central nervous system (cns) injury, tissue damage occurs via primary and secondary mechanisms. despite many atte...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

embryonic stem cells (escs) are undifferentiated cells which are typically derived from the inner cell mass of a blastocyst-stage embryo. in culture, these cells have the capacity to self-renew in an undifferentiated state but also may differentiate into cell types representing the three embryonic germ lineages, thus revealing their pluripotent potential. these cells could be of value for creat...

introduction: the value of embryonic stem-like cells in cloning is obvious. production of cloned animals can be achieved by introduction of these cells into a tetraploid embryo. tetraploid embryo is used as a feeder for development of embryonic stem-like cells and can be produced in vitro by electro-fusion of 2-cell embryos. the aim of this study was to ass,essthe effect of voltage alteration a...

BACKGROUND Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy. OBJECTIVE This study presents an economical and simple co-culture system for pluripotent stem cell...

The embryonic stem cell-like gene expression signature has been shown to be associated with poorly differentiated aggressive human tumors and has attracted great attention as a potential target for future cancer therapies. Here, we investigate the potential of the embryonic stem cell signature as molecular target for the therapy and the strategy to suppress the embryonic stem cell signature. Th...

objective: mir-302-367 is a cluster of polycistronic micrornas that are exclusively expressed in embryonic stem (es) cells. the mir-302-367 promoter is functional during embryonic development but is turned off in later stages. motivated by the cancer stem cell hypothesis, we explored the potential expression of mir-302 in brain tumor cell lines. materials and methods: in the present experimenta...